Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

By J. Kowal, Guillaume Arras, Marina Colombo, M. Jouve, Jakob Paul Morath, Bjarke Primdal-Bengtson, F. Dingli, D. Loew, M. Tkach, C. Théry. Proceedings of the National Academy of Sciences of the United States of America, 2016

3,103 citations recorded by the source corpus.

Subfields: Cancer Research, Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/13e18c07d6c386cdee1b9d07f88a8365347212b2

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Citations in the corpus

Extracellular Vesicles: Unique Intercellular Delivery Vehicles. (2017, methodology): “Using such a density gradient method for proteomic characterization of EVs from dendritic and other cell types, it was found that syntenin-1, EHD4, annexin XI and ADAM10 were unique to sEVs, while actinin-4 and mitofilin were highly abundant on lEVs [7].”
Adipose Tissue Macrophage-Derived Exosomal miRNAs Can Modulate In Vivo and In Vitro Insulin Sensitivity. (2017, neutral): “…http://dx.doi.org/10.1016/j.cell.2017.08.035 identified these extracellular vesicles as Exos with a diameter of 30-100 nanometers by electromicroscopy, NanoSight analysis, and measurement of the exosomal membrane markers TSG101, syntenin 1, CD63, and CD9 (Kowal et al., 2016; Zhang et al., 2015a).”
The biology and function of exosomes in cancer. (2016, background): “The definition of the term exosomes will likely continue to evolve over time in the scientific literature with ongoing efforts to better track their endocytic origin and distinguish them from other microvesicles (6).”
The biology, function, and biomedical applications of exosomes (2020, neutral): “Proteomic analyses of EVs have revealed the marker heterogeneity of exosomes, cautioning their utility in experimental design usingmarkerdetermined purification methodologies (23).”
A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option? (2020, background): “This is done by gradually separating the pellet and supernatant at increasing speeds: 300–400× g for 10 min, then 2000× g, and finally 10,000× g, to isolate a supernatant containing a relatively high concentration of exosomes albeit still contaminated with microvesicles, lipoprotein moieties, and ot”