Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds

By S. Levenberg, N. Huang, E. Lavik, A. Rogers, J. Itskovitz‐Eldor, Robert Langer. Proceedings of the National Academy of Sciences of the United States of America, 2003

1,214 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/5ff2790a84909ca06de7f3620dfd22bed6cc5c2d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. K. Leong
2. N. Cameron
3. A. Bishop
4. M. Tibbitt
5. K. Anseth
6. S. Levenberg
7. H. Rippon
8. Jason A. Burdick

In Molecular Biology

1. A. Ramachandran
2. S. Levenberg
3. Christoph Borner
4. H. Jaeschke
5. A. Bishop
6. K. Leong
7. K. Anseth
8. Ute Albrecht

In Surgery

1. A. Ramachandran
2. H. Jaeschke
3. K. Leong
4. M. Tibbitt
5. K. Anseth

Citations in the corpus

• Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME (2013, positive): “Moreover, large research programs have been initiated to differentiate human hepatocyte-like cells from stem or precursor cells (Fletcher et al. 2008; Agarwal et al. 2008; Cai et al. 2007).”
• Embryonic stem cells (2004, positive): “…on a polymeric scaffold designed for the support of complex tissue structures rather than via EB formation, and these were observed to form structures with the characteristics of neural tissues, cartilage and liver, as well as a network of blood vessel-like tubules (Levenberg et al. 2003).”
• Engineering vascularized skeletal muscle tissue (2005, methodology): “Porous sponges composed of 50% PLLA and 50% PLGA were fabricated as describe”
• Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. (2007, background): “Although the concentration of 1μM of RA has been shown to be sufficient in inducing neuronal differentiation in embryonic stem cells[27] and neuronal stem cells[28], a concentration of 30μM RA is necessary to achieve the neuronal differentiation of hMSCs [29].”
• Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture (2009, methodology): “To this end, cells have been encapsulated within microporous (Levenberg et al., 2003; Shea et al., 1999; Yim and Leong, 2005), nanofibrous (Semino et al., 2003; Silva et al., 2004), and hydrogel scaffolds (Peppas et al., 2006).”
• Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells (2004, neutral): “Embryoid bodies were formed and then trypsinized after 6”