PCR technology for screening and quantification of genetically modified organisms (GMOs)

By Arne Holst-Jensen, Sissel Beate Rønning, Astrid Løvseth, Knut G. Berdal. Analytical and Bioanalytical Chemistry, 2003

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Detection and traceability of genetically modified organisms in the food production chain. (2004, methodology): “New strategies to improve the availability of reference materials have been explored, including the use of material from contaminated products (Holst-Jensen et al., 2003) and development of plasmids as reference materials (Taverniers et al., 2001; Kuribara et al., 2002).”
Kernel lot distribution assessment (KeLDA): a study on the distribution of GMO in large soybean shipments (2006, neutral): “to standard real-time PCR procedures [20, 21].”
A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production (2007, background): “…matrices, the powder CRMs may not be suitable reference material for GM crop quantification given that DNA and protein levels may vary on a weight percent basis due to factors such as variety and growing conditions, which could produce error when analyzing test samples (Holst-Jensen et al., 2003).”
Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method (2010, methodology): “In particular, conventional and real-time quantitative polymerase chain reaction (PCR) methods have been widely accepted as standards for identifying and determining the GM contents in food and feeds because of their high efficiency, sensitivity, and stability (Bellocchi et al. 2008; Holst-Jensen et”