Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes.

By Anna Válóczi, Csaba Hornyik, N. Varga, J. Burgyán, Sakari Kauppinen, Z. Havelda. Nucleic Acids Research, 2004

821 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/5ed64974362068c8f5ad78d46d17bcfb5bdd3581

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Vahap Eldem
2. Turgay Unver
3. S. Okay
4. S. Sen
5. Jin Wang
6. Jinyun Chen
7. Sakari Kauppinen
8. Asli Silahtaroglu

In Molecular Biology

1. Vahap Eldem
2. S. Okay
3. Turgay Unver
4. S. Sen
5. Jin Wang
6. Jinyun Chen
7. Sakari Kauppinen
8. Asli Silahtaroglu

In Plant Science

1. Vahap Eldem
2. Turgay Unver
3. S. Okay

Citations in the corpus

• MicroRNA detection by northern blotting using locked nucleic acid probes (2008, methodology): “To avoid this problem, we have developed a new method based on LNA-modified oligonucleotide probe”
• Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies (2006, background): “Developing high-throughput microarray platforms that achieve highly sensitive and accurate discrimination of all miRNAs, including the most homologous of paralogs, remains a technical challenge, although recent studies suggest that further microarray detection specificity may be achieved by employin”
• Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning (2006, neutral): “Blots were hybridized with end-labeled oligonucleotide probe”
• microRNA as Biomarkers and Diagnostics (2016, methodology): “…Vitro Diagnostics In the past few years, various approaches have been used to detect miRNAs, including Northern blot analysis (Sempere et al., 2004; Valoczi et al., 2004), in situ hybridization analysis (Kloosterman et al., 2006), real-time PCR analysis (Chen et al., 2005; Wang et al., 2009),…”
• Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver (2007, positive): “LNA probes were labelled and hybridized to the membrane as described (23) with the following modifications: pre-hybridization and hybridization solutions contained 50% formamide, 0.”
• Plant microRNAs: new players in functional genomics (2013, positive): “Valoczi et al. (2004) made some alteration in northern blotting by using modified oligonucleotides using locked nucleic acid (LNA), which increases the method’s sensitivity for the detection of mature miRNAs by at least 10 times (Valoczi et al. 2004; Varallyay et al. 2008).”
• Modulating Hox gene functions during animal body patterning (2005, methodology): “Another method to detect miRNA expression patterns involves the use of LOCKED-NUCLEIC-ACID OLIGONUCLEOTIDE PROBES”
• miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis (2020, background): “miRNAs can be detected using various specific and sensitive approaches, including Northern blot analysis [294,295], in situ hybridization [296], real-time PCR [95,297], miRNA microarray [298,299] and next-generation sequencing (NGS) [300], and some of them are already used as diagnostic or prognosti”