DNMT1 and DNMT3b cooperate to silence genes in human cancer cells

By Ina Rhee, K. Bachman, B. Park, K. Jair, R. Yen, Kornel E. Schuebel, H. Cui, Andrew P. Feinberg, Christoph Lengauer, Kenneth W. Kinzler and 2 more. Nature, 2002

1,301 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5e79bf2ef7815490141d62c9768d68f807a4c148

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Citations in the corpus

A stem cell–like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing (2007, methodology): “Tera-1, Tera-2 and HCT-116 (ATCC) cells were maintained in McCoy's 5A medium supplemented with 10% FBS and grown at 37 °C under 5% CO 2 , as were HCT 116 cells in which both DNA methyltransferases 1 and 3b are genetically deleted (DKO cells”
Chemoprevention of colon cancer by calcium, vitamin D and folate: molecular mechanisms (2003, result): “In contrast with these findings, however, somatic inactivation of DNMT1 by homologous recombination in a human colorectal carcinoma cell line, although leading to a marked decrease in DNMT1 activity, did not significantly affect the methylation status of tumour-suppressor gene”
Epigenetic Determinants of Cancer. (2016, background): “Genetic disruption studies of DNMTs in cultured colon cancer cells indicate that the maintenance of most DNA methylation, including at hypermethylated promoters and its attendant gene silencing, requires both DNMT1 and DNMT3b (Rhee et al. 2000, 2002).”
Dnmt1 and Dnmt3a are required for the maintenance of DNA methylation and synaptic function in adult forebrain neurons (2010, background): “For example, DNMT3B and DNMT1 are known for their cooperative role in maintaining DNA methylation in colon cancer cell”
Gene Body Methylation can alter Gene Expression and is a Therapeutic Target in Cancer (2014, methodology): “Wenext monitored global DNAmethylation and gene expression profiles in two HCT116 derivative cell lines with genetic knockouts for DNMT3B (3BKO) or for DNMT1 (1KO) (Rhee et al., 2000, 2002) to determine the specific DNMTs responsible for restoring the demethylation after 5-Aza-CdR treatment.”
Genetic unmasking of an epigenetically silenced microRNA in human cancer cells. (2007, background): “DKO cells show a drastic reduction of DNMT activity, 5-methylcytosine DNA content, and, most important, a release of gene silencing associated with CpG island hypomethylation (11, 13).”
Analysis of repetitive element DNA methylation by MethyLight (2005, background): “Sat2 hypomethylation had been described for both the single and double knockout HCT116 cells when compared with wild-type HCT116 cells (51).”
Validation of a DNA methylation microarray for 450,000 CpG sites in the human genome (2011, methodology): “Most importantly, the HCT-116 double-knockout cells for DNMT1 and DNMT3b (DKO cells) have minimal DNA methyltransferase activity, a 95% reduction in 5-methylcytosine content, demethylation of repeated sequences and loss of imprinting at the insulin-like growth factor II locus.(29) Interestingly, DKO”
DNA methylation and cancer. (2004, background): “Genetic disruption of both DNMT1 and DNMT3b in a colorectal cell line reduced DNA methylation and resulted in the loss of insulin-like growth factor II imprinting, abrogation of silencing of the tumor suppressor gene p16, and growth suppression.(19) Multiple intestinal neoplasia mice carry a germ-li”
Mechanisms of drug combinations: interaction and network perspectives (2009, background): “An example of actions on different targets is provided by the anticancer combination of cisplatin and topoteca”
Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer. (2008, methodology): “We also analyzed HCT116 cells, in which the DNMT genes DNMT1 and DNMT3B were genetically disrupted (DKO2 cells), thereby abrogating DNA methylation (18, 20).”
Chromatin modification and epigenetic reprogramming in mammalian development (2002, background): “cgi?l=1789">DNMT3B have been shown to have overlapping functions in maintaining genome-wide methylation pattern”
Highly integrated single-base resolution maps of the epigenome in Arabidopsis. (2008, neutral): “In higher eukaryotes, DNA methylation is involved in myriad essential processes, including embryogenesis, genomic imprinting, and tumorigenesis in mammals, and in transposon silencing and gene regulation in plants (Bestor, 2000; Li et al., 1992; Lippman et al., 2004; Rhee et al., 2002; Zhang et al.,”
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells (2003, background): “, whereas HCT116 clones with inactivation of both DNMT1 and DNMT3B showed much lower levels of DNA methylation, suggesting that the two enzymes are highly cooperativ”