Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery.

By M. Lo, A. Aulabaugh, G. Jin, R. Cowling, J. Bard, M. Malamas, G. Ellestad. Analytical Biochemistry, 2004

678 citations recorded by the source corpus.

Subfields: Computational Theory and Mathematics, Molecular Biology, Physical and Theoretical Chemistry

View Paper: https://www.semanticscholar.org/paper/5bbb3b0fd1831814ee4ae4327de126dff95ba56a

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In Molecular Biology

1. P. Czabotar
2. A. Strasser
3. Ian J. Majewski
4. Warren S. Alexander
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6. Jerry M. Adams

Citations in the corpus

• Thermofluor-based high-throughput stability optimization of proteins for structural studies. (2006, methodology): “The thermofluor assay can be performed in a commercially available real-time PCR instrument [4], where ther-”
• The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. (2013, background): “…ligand binding confers thermal stability to the protein, resulting in an increase in the melting temperature that can be measured by means of the dye Sypro Orange, which fluoresces upon binding to the hydrophobic surfaces exposed during protein denaturation (Lo et al., 2004; Lucet et al., 2013).”