Thermofluor-based high-throughput stability optimization of proteins for structural studies.

By U. Ericsson, B. Hallberg, G. DeTitta, N. Dekker, Pär Nordlund. 2006

891 citations recorded by the source corpus.

Subfields: Materials Chemistry, Molecular Biology, Spectroscopy

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In Molecular Biology

1. Thomas J. Magliery
2. E. Krol
3. Anke Becker
4. Jason J. Lavinder
5. B. Sullivan

Citations in the corpus

• Structural Basis of Dual Specificity of Sinorhizobium meliloti Clr, a cAMP and cGMP Receptor Protein (2023, methodology): “Preceding the purification, the size exclusion buffer was optimized as described using SYPRO orange dye (84).”
• Protein stability by number: high-throughput and statistical approaches to one of protein science’s most difficult problems (2011, positive): “Most laboratory implementations of DSF, which is used extensively to optimize buffer conditions for crystallography [38,39], use real-time PCR machines that typically lack filter sets in the blue.”