Targeted screening for induced mutations

By C. McCallum, Luca Comai, E. Greene, Steven Henikoff. Nature Biotechnology, 2000

740 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/5634f677aa4cd0fa218b508e1416ed174c558a15

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. C. Mba
2. M. Germanà
3. M. Maluszynski
4. E. Blumwald
5. J. Dalton‐Morgan
6. P. Sharp
7. Chongmei Dong
8. Kate Vincent

In Plant Science

1. C. Mba
2. M. Germanà
3. M. Maluszynski
4. Jose Luis Araus
5. G. Slafer
6. Conxita Royo
7. M. D. Serret
8. E. Blumwald

Citations in the corpus

• Genome Editing of Plants (2017, background): “Four independent mutations were identified from a pool of approximately 400 M2 mutants (McCallum et al., 2000).”
• Arabidopsis Map-Based Cloning in the Post-Genome Era (2002, methodology): “When using reverse genetic approaches, such as tilling for point mutations (McCallum et al., 2000) or searching for T-DNA insertion mutations (Sussman et al., 2000), one starts with a gene of interest, finds a mutation in that gene, and then looks for a phenotype.”
• Targeting induced local lesions IN genomes (TILLING) for plant functional genomics. (2000, background): “We have introduced a new reverse genetic strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid mutational screening to discover induced lesions (McCallum et al., 2000).”
• Production of novel allelic variation for genes involved in starch biosynthesis through mutagenesis (2010, background): “identification, by mismatch cleavage of polymerase chain reaction (PCR) products, of single base nucleotide polymorphisms (SNPs) in specific genes within a population of plants, produced by treating seeds or pollen with a chemical mutagen (McCallum et al. 2000), or in diversity collections (Comai et”
• A Modified TILLING Method for Wheat Breeding (2009, methodology): “By comparison, EMS mutagenesis in Arabidopsis produced a 1 in 170 kb mutation frequency (Greene et al., 2003); barley had 1 in 1000 kb (Caldwell et al., 2004); and rice had 1 in 300 to 600 kb (Till et al., 2007).”
• SNP frequency and allelic haplotype structure of Beta vulgaris expressed genes (2001, background): “In addition, SNPs leading to a nonconservative amino acid exchange may be correlated with a phenotypic effect, which allows the development of reverse genetic screens based on chemical mutagenesis (McCallum et al. 2000).”
• Large-scale discovery of induced point mutations with high-throughput TILLING. (2003, methodology): “As the TILLING methodology identifies polymorphisms regardless of phenotypic consequence, the mutations described here represent the most comprehensive data set on EMS mutagenesis in Arabidopsis to date.”
• Genome diversity and gene haplotypes in the grapevine (Vitis vinifera L.), as revealed by single nucleotide polymorphisms (2004, background): “They have a high level of potential automation, which increases mapping efficiency Landgreen et al. 1998 , and in some cases correlate with non-conservative amino acid changes which lead to phenotypic variation and with the consequent development of reverse genetic screens McCallum et al. 2000 .”
• New developments in fiber hemp (Cannabis sativa L.) breeding (2015, background): “…alter the functionality of genes and selected mutant genotypes re directly useful as breeding material (Comai et al., 2004; Elshire t al., 2011; McCallum et al., 2000; Metzker, 2010). cknowledgments The authors gratefully acknowledge funding from the European nion consortia FIBRA (project ID…”
• Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination (2012, background): “In addition, direct estimates confirm that base substitution rates are comparable for Arabidopsis seeds soaked in EMS [6,7] and EMS-fed Drosophila males [8], and approximately similar rates were found in a reverse-genetic screen of zebrafish progeny exposed to N-ethyl-N-nitrosourea (ENU) [9].”
• Induced Mutations Unleash the Potentials of Plant Genetic Resources for Food and Agriculture (2013, methodology): “The reverse genetic technique, TILLING (Targeting Induced Local Lesions IN Genomes [65], that was aptly characterized “Traditional Mutagenesis Meets Functional Genomics” [66], permits the high throughput querying of putative mutants for point mutation events in specific genomic regions.”
• Denaturing high‐performance liquid chromatography: A review (2001, background): “From a total of ~2 Mb of DNA sequence screened in the chromomethylase genes CMT2 and CMT3 [McCallum et al., 2000a], 13 mutations, nine of which were redundant, were detected and confirmed by sequencing.”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “thaliana, CMT genes are generally expressed at moderate levels in all organs of the plant (McCallum et al., 2000).”