Targeting induced local lesions IN genomes (TILLING) for plant functional genomics.

By C. McCallum, Luca Comai, E. Greene, Steven Henikoff. Plant Physiology, 2000

897 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/a9af0e570a5296524640ba02a15804a915bbf81d

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Citations in the corpus

Physiology and molecular biology of stress tolerance in plants (2006, methodology): “These populations are screened for mutations using denaturing high performance liquid chromatography (DHPLC) (McCallum et al., 2000) or endonuclease, which would specifically cleave mismatches in a heteroduplex between wild type and mutant (Colbert et al., 2001).”
Abscisic Acid Signaling in Seeds and Seedlings Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010441. (2002, background): “Recently, “reverse-genetics” studies have been used to test a specific gene’s functional role; these studies use either screening of large mutagenized populations (McCallum et al., 2000; Parinov and Sundaresan, 2000) or a transgenic approach to overexpress or disrupt a target gene.”
A Modified TILLING Method for Wheat Breeding (2009, methodology): “Th e TILLING method is useful for both functional genomics as demonstrated in Arabidopsis (McCallum et al., 2000b) and crop improvement as demonstrated in wheat (Triticum aestivum L.”
Large-scale discovery of induced point mutations with high-throughput TILLING. (2003, methodology): “Additional Blocks may be generated using SIFT (Ng and Henikoff 2001), which is incorporated into our automated ordering system.”
The zebrafish (Danio rerio) as a model organism, with emphasis on applications for finfish aquaculture research (2014, background): “Other strategies such as the Targeting Induced Local Lesions IN Genomes (TILLING) have been very popular as it rapidly allows the identification of large numbers of alleles from mutant libraries (McCallum et al. 2000; Geisler 2011).”
The Sequence of 1504 Mutants in the Model Rice Variety Kitaake Facilitates Rapid Functional Genomic Studies (2017, background): “scan amplicons from a large set of mutants for each use (McCallum et al., 2000).”
Development of Genetic and Genomic Research Resources for Brachypodium distachyon, a New Model System for Grass Crop Research (2008, positive): “Th is includes overexpression of candidate genes in transgenic plants, directed knock-down or targeted mutation of candidate genes by TILLING (McCallum et al., 2000), delete-a-gene (Li and Zhang, 2002), RNAi or artifi cial microRNAs (amiRNAs) (Schwab et al., 2006), development of a full-length cDNA ”
Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination (2012, background): “In addition, direct estimates confirm that base substitution rates are comparable for Arabidopsis seeds soaked in EMS [6,7] and EMS-fed Drosophila males [8], and approximately similar rates were found in a reverse-genetic screen of zebrafish progeny exposed to N-ethyl-N-nitrosourea (ENU) [9].”
Predicting deleterious amino acid substitutions. (2001, background): “2000) or in genome-wide random mutagenesis projects (Bentley et al. 2000; Chen et al. 2000; McCallum et al. 2000).”
Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping (2007, methodology): “TILLING (Targeting Induced Local Lesions IN Genomes) and its derivative EcoTILLING were developed as techniques to identify SNPs in a population of mutants and ecotypes, respectively (McCallum et al. 2000; Comai et al. 2004).”
Genomics of Phaseolus Beans, a Major Source of Dietary Protein and Micronutrients in the Tropics (2008, background): “a gene of interest from a large mutagenesis population (McCallum et al. 2000).”
Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes (2006, methodology): “This technique combines chemical mutagenesis with a powerful screening method for potential mutations (Gilchrist & Haughn, 2005; Henikoff et al., 2004; McCallum et al., 2000).”