Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling

By R. Stracke, Hirofumi Ishihara, G. Huep, A. Barsch, Frank Mehrtens, Karsten Niehaus, Bernd Weisshaar. The Plant Journal, 2007

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The genes MYB11, MYB12 and MYB111 share significant structural similarity and form subgroup 7 of the Arabidopsis thaliana R2R3-MYB gene family. To determine the regulatory potential of these three transcription factors, we used a combination of genetic, functional genomics and metabolite analysis approaches. MYB11, MYB12 and MYB111 show a high degree of functional similarity and display very similar target gene specificity for several genes of flavonoid biosynthesis, including CHALCONE SYNTHASE, CHALCONE ISOMERASE, FLAVANONE 3-HYDROXYLASE and FLAVONOL SYNTHASE1. Seedlings of the triple mutant myb11 myb12 myb111, which genetically lack a complete subgroup of R2R3-MYB genes, do not form flavonols while the accumulation of anthocyanins is not affected. In developing seedlings, MYB11, MYB12 and MYB111 act in an additive manner due to their differential spatial activity; MYB12 controls flavonol biosynthesis mainly in the root, while MYB111 controls flavonol biosynthesis primarily in cotyledons. We identified and confirmed additional target genes of the R2R3-MYB subgroup 7 factors, including the UDP-glycosyltransferases UGT91A1 and UGT84A1, and we demonstrate that the accumulation of distinct and structurally identified flavonol glycosides in seedlings correlates with the expression domains of the different R2R3-MYB factors. Therefore, we refer to these genes as PFG1–3 for ‘PRODUCTION OF FLAVONOL GLYCOSIDES’.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/54bb6d9ee65d2abdacddb8a5f45e0af4549f1c33

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Citations in the corpus

The Jasmonate-ZIM-Domain Proteins Interact with the WD-Repeat/bHLH/MYB Complexes to Regulate Jasmonate-Mediated Anthocyanin Accumulation and Trichome Initiation in Arabidopsis thaliana[C][W] (2011, background): “…members EGL3 and TT8 (Pires and Dolan, 2010), the R2R3 MYB factors, including MYB75 and its closely relatedmembersMYB90,MYB113,MYB114, andGL1 (Stracke et al., 2001), and a WD-repeat protein, TTG1, form WD-repeat/bHLH/MYB complexes to mediate diverse plant responses (Zimmermann et al., 2004;…”
Negative Regulation of Anthocyanin Biosynthesis in Arabidopsis by a miR156-Targeted SPL Transcription Factor[W][OA] (2011, background): “These two MYBs are positive regulators of CHS and CHI (Stracke et al., 2007), and the low levels of MYB12 and MYB111 transcripts might explain why CHI and CHS levels were decreased in Pro35S:MIR156 plants as well.”
Flavonoids as Important Molecules of Plant Interactions with the Environment (2014, background): “MYB regulators are divided into four types: MYB1R, R2R3-MYB, R1R2R3 MYB (MYB 3R) and 4RYB [124,125].”
Germin and Germin-like Proteins: Evolution, Structure, and Function (2008, background): “To determine the global regulatory potential of these three transcription factors, a combination of genetic, functional genomics and metabolite analysis approaches was used (Stracke et al., 2007).”
Biosynthesis and Regulation of Phenylpropanoids in Plants (2017, background): “AtFLS, AtCHS, AtCHI, AtF3H and AtF30H are activated by independent MYB TFs, such as AtMYB12/ PFG1 (PRODUCTION OF FLAVONOL GLYCOSIDES 1), AtMYB11/PFG2 and AtMYB111/PFG3 that belong to subgroup 7 (Figure 2) (Stracke et al., 2007, 2010).”
Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. (2011, background): “…regions has been shown to play a prominent role in the evolution of Arabidopsis MYB genes, in particular sub-groups 6 and 7 (Cannon et al., 2004; Stracke et al., 2007; Matus et al., 2008), and also in poplar (Populus trichocarpa), in which more than 35% of the R2R3- MYB genes are part of tandem…”
An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae (2010, background): “The MYB TF superfamily illustrates how a relatively small family in animal genomes (3 members of this TF type in the human genome by BLAST match) controlling cell division and differentiation has become the most abundant TF group in plants [48] with diverse functions in hormone response [49], growth”
Flavonoids: biosynthesis, biological functions, and biotechnological applications (2012, background): “Studies in a wide range of species, such as Ligustrum vulgare, Vitis vinifera, petunia, and Arabidopsis have provided new evidence that UV light induces the synthesis of flavonol compounds (Ryan et al., 2002; Berli et al., 2010; Stracke et al., 2010; Agati et al., 2011; Kusano et al., 2011).”
Cooperative Regulation of Flavonoid and Lignin Biosynthesis in Plants (2021, background): “From this, MYB proteins are classified into four major types such as 1R-MYB, 2R-MYB, 3R-MYB, and 4R-MYB proteins, of which the R2R3-MYBs are the most common type in plants (Urao et al., 1993; Stracke et al., 2001; Du et al., 2009; Dubos et al., 2010; Du et al., 2012; Zhou et al., 2019).”
Phenylpropanoid biosynthesis. (2010, neutral): “…helix-loop-helix bHLH and MybR2R3-type at F M R P /U S P /B IB LIO T E C A C E N T R A L on A ugust 7, 2011 m plant.oxfordjournals.org D ow nloaded from transcription factors has been the topic of many reports, such as Stracke et al. (2007), and several reviews (Broun, 2005; Allan et al., 2008).”
Molecular characterization of EgMYB1, a putative transcriptional repressor of the lignin biosynthetic pathway (2007, background): “One likely hypothesis is that the regulation of this complex metabolic pathway and the levels of the various end-products accumulated in specific cells, tissues or organs can be explained by modulating the expression of several of these MYB proteins [39,40].”
MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants. (2015, background): “…24 CoA shikimate/quinate transferase), C3H (encoding p-coumaroyl ester 3-hydroxylase) 25 and HQT (encoding hydroxycinnamoyl CoA quinate transferase) (Aharoni et al., 2001; 26 Stracke et al., 2007; Deluc et al., 2008; Luo et al., 2008; Paolocci et al., 2011; 27 Vimolmangkang et al., 2013).”
Comparative metabolomics and transcriptomics of plant response to Tomato yellow leaf curl virus infection in resistant and susceptible tomato cultivars (2014, background): “In Arabidopsis, the genes in the flavonol pathway are regulated by the transcription factors MYB11, MYB12 and MYB111 (Stracke et al. 2007), while the DFR and ANS genes leading to anthocyanin biosynthesis are regulated by the transcription factors MYB75 and MYB90 (Tohge et al.”
Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids (2013, methodology): “…and MYB111/PFG3 regulate expression of the four early biosynthetic genes chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), and flavonol synthase (FLS) (Stracke et al., 2007, 2010b), whereas the anthocyanin regulators MYB75/PAP1 (PRO-”