LC3 and GATE‐16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis

By H. Weidberg, E. Shvets, Tomer Shpilka, F. Shimron, V. Shinder, Zvulun Elazar. EMBO Journal, 2010

679 citations recorded by the source corpus.

Subfields: Epidemiology, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/544982060cb0cc17bc0fb5dc7048a50f29b62040

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Citations in the corpus

Atg8 family LC3/GABARAP proteins are crucial for autophagosome–lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation (2016, background): “Consistent with reported roles for Atg8s in expanding autophagosomal membranes (Nakatogawa et al., 2007; Weidberg et al., 2010), we observed smaller autophagosomes in hexa KO cells (Fig.”
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2012, neutral): “The LC3 proteins are involved in phagophore formation, with participation of GABARAP subfamily members in later stages of autophagosome formation, in particular phagophore elongation and closure.(142) Some evidence, however, suggests that at least in certain cell types the LC3 subfamily may be dispe”
Autophagy and the integrated stress response (2010, positive): “In mammals, four Atg4 (Atg4A-D) and at least six orthologs of Atg8 exist, among which LC3B (hereafter referred to simply as LC3), GABARAP, and GATE16 have been most studied (Weidberg et al., 2010).”
Guidelines for the use and interpretation of assays for monitoring autophagy (2012, background): “The LC3 proteins are involved in phagophore formation, with participation of GABARAP subfamily members in later stages of autophagosome formation, in particular phagophore elongation and closure.(110) Nevertheless, in most published studies, LC3 has been the primary Atg8 homolog examined in mammalia”
The regulation of autophagy – unanswered questions (2011, background): “A recent study has demonstrated that LC3 is involved in phagophore membrane expansion, whereas the GABARAP family is required for autophagosome maturation (Weidberg et al., 2010).”
Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy. (2014, background): “Thus, attached to the lipid, Atg8 tethers lipid bilayers (Nakatogawa et al., 2007), a function associated with its N-terminal a helices (Weidberg et al., 2011); recruits additional components of the autophagic machinery to the phagophore (Alemu et al., 2012; Colecchia et al., 2012; Popovic et al.,…”
An Overview of Autophagy: Morphology, Mechanism, and Regulation (2014, background): “Whereas both subfamilies can localize with autophagosomes (64), it has been proposed that they function at different steps in phagophore elongation and completion, with the LC3 subfamily acting before the GABARAP subfamily (146).”
Ubiquitination and selective autophagy (2012, background): “It appears that both subfamilies are required for autophagy and that members of both subfamilies contribute to different aspects of autophagosome biogenesis.(51)”