Solvent content of protein crystals.

By B. Matthews. 1968

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Citations in the corpus

Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule. (2000, background): “At this stage, crystal packing analysis showed that the crystal contained twice as many FNR as Fd molecules, with a VM of 1.96 AÊ 3 Daÿ1 (Matthews, 1968).”
X-ray absorption by macromolecular crystals: the effects of wavelength and crystal composition on absorbed dose (2004, background): “The contents of the unit cell in macromolecular crystallography are usually well de®ned, since the sequence and thus composition of the macromolecule is known, and the proportion of solvent may be estimated (Matthews, 1968).”
Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra α-helical domain (2002, background): “A Matthews coef®cient (Matthews, 1968 ) of 2.3 A Ê 3 /Da and a solvent content of 51% were obtained assuming six molecules in the asymmetric unit.”
An approach to rapid protein crystallization using nanodroplets (2002, unknown): “This calculation assumes that the crystals contain about 50% solvent and also assumes that most of the protein in a drop contributes to the formation of a single crystal (Matthews, 1968).”
Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein–nucleic acid complex crystals (2003, methodology): “Although this idea has been assumed for a number of years (Matthews 1968, 1976; Podjarny et al. 2002), it is substantiated by statistical validation of the observed data.”
Crystal structure of a Cbtx–AChBP complex reveals essential interactions between snake α‐neurotoxins and nicotinic receptors (2005, background): “They belonged to the orthorhombic space group C2221 with unit cell dimensions a¼ 162.6 Å, b¼ 313.4 Å, and c¼ 106.5 Å, giving a Vm value of 4.1 Å3/Da (70% solvent) for one pentameric Cbtx–AChBP complex in the asymmetric unit (Matthews, 1968).”
Structures of bovine, equine and leporine serum albumin. (2012, background): “The crystal of BSA belonged to a centred monoclinic system in space group C2; the Matthews coefficient (Matthews, 1968) indicated the presence of two monomers in the asymmetric unit.”