Quantifying memory CD8 T cells reveals regionalization of immunosurveillance
By Elizabeth M. Steinert, J. Schenkel, Kathryn A. Fraser, Lalit K. Beura, Luke S Manlove, B. Igyártó, P. Southern, D. Masopust. Cell, 2015
653 citations recorded by the source corpus.
Summary Memory CD8 T cells protect against intracellular pathogens by scanning host cell surfaces, thus infection detection rates depend on memory cell number and distribution. Population analyses rely on isolation from whole organs and interpretation is predicated on presumptions of near complete cell recovery. Paradigmatically, memory is parsed into central, effector, and resident subsets, ostensibly defined by immunosurveillance patterns, but in practice identified by phenotypic markers. Because isolation methods ultimately inform models of memory T cell differentiation, protection, and vaccine translation, we tested their validity via parabiosis and quantitative immunofluorescence microscopy of a mouse memory CD8 T cell population. We report three major findings: lymphocyte isolation fails to recover most cells and biases against certain subsets, residents greatly outnumber recirculating cells within nonlymphoid tissues, and memory subset homing to inflammation does not conform to previously hypothesized migration patterns. These results indicate that most host cells are surveyed for reinfection by segregated residents rather than by recirculating cells that migrate throughout the blood and body.
Subfields: Immunology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/52cb5692c6b5116213655384815bd3e046b782a1
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Citations in the corpus
- The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans (2021, background): “This approach enables an estimate of true cell type proportions since not every cell type survives dissociation with equal efficiency (15).”
- Human tissue-resident memory T cells are defined by core transcriptional and functional signatures in lymphoid and mucosal sites (2017, background): “In mice, tissue retention demonstrated by parabiosis (Jiang et al., 2012; Steinert et al., 2015) and in vivo antibody labeling (Anderson et al., 2014; Turner et al., 2014) identified phenotypic markers associated with tissue residence, including CD69 and CD103.”
- Human T cell development, localization, and function throughout life (2018, background): “…in diverse types of tissues including barrier sites, mucosal tissues, liver, salivary glands, and brain, where they are retained and do not recirculate as shown by parabiosis and in vivo labeling studies (Jiang et al., 2012; Mueller and Mackay, 2016; Steinert et al., 2015; Teijaro et al., 2011).”