Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.

By Tian Xia, M. Kovochich, Jonathan A. Brant, Matt Hotze, J. Sempf, T. Oberley, C. Sioutas, Joanne I. Yeh, M. Wiesner, A. Nel. Nano letters (Print), 2006

1,892 citations recorded by the source corpus.

Nanomaterial properties differ from those bulk materials of the same composition, allowing them to execute novel activities. A possible downside of these capabilities is harmful interactions with biological systems, with the potential to generate toxicity. An approach to assess the safety of nanomaterials is urgently required. We compared the cellular effects of ambient ultrafine particles with manufactured titanium dioxide (TiO2), carbon black, fullerol, and polystyrene (PS) nanoparticles (NPs). The study was conducted in a phagocytic cell line (RAW 264.7) that is representative of a lung target for NPs. Physicochemical characterization of the NPs showed a dramatic change in their state of aggregation, dispersibility, and charge during transfer from a buffered aqueous solution to cell culture medium. Particles differed with respect to cellular uptake, subcellular localization, and ability to catalyze the production of reactive oxygen species (ROS) under biotic and abiotic conditions. Spontaneous ROS production was compared by using an ROS quencher (furfuryl alcohol) as well as an NADPH peroxidase bioelectrode platform. Among the particles tested, ambient ultrafine particles (UFPs) and cationic PS nanospheres were capable of inducing cellular ROS production, GSH depletion, and toxic oxidative stress. This toxicity involves mitochondrial injury through increased calcium uptake and structural organellar damage. Although active under abiotic conditions, TiO2 and fullerol did not induce toxic oxidative stress. While increased TNF-alpha production could be seen to accompany UFP-induced oxidant injury, cationic PS nanospheres induced mitochondrial damage and cell death without inflammation. In summary, we demonstrate that ROS generation and oxidative stress are a valid test paradigm to compare NP toxicity. Although not all materials have electronic configurations or surface properties to allow spontaneous ROS generation, particle interactions with cellular components are capable of generating oxidative stress.

Subfields: Biomedical Engineering, Materials Chemistry, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/523c8a724fcdb9f51c3aa8f0896a660dfe5fe3c4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Yury Gogotsi
2. K. Mahmoud
3. J. Zink
4. M. Liong
5. Yongsheng Chen
6. O. Farokhzad
7. Huan Meng
8. Tian Xia

In Materials Chemistry

1. Yury Gogotsi
2. K. Mahmoud
3. Chang E. Ren
4. A. Nel
5. Diana Anderson
6. Kashif Rasool
7. Tian Xia
8. Vyom Sharma

In Organic Chemistry

1. Yury Gogotsi
2. M. Monteiro
3. Ning Li
4. A. Nel
5. Paul Borm
6. W. D. De Jong
7. M. Wiesner

Citations in the corpus

• Quantum dots versus organic dyes as fluorescent labels (2008, background): “To avoid cytotoxic materials in general, possible alternatives to classical QD labels could be III/V group (binary or ternary) semiconductors such as InP or InGa”
• The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. (2011, result): “suggests that the generation of ROS inmacrophages was an indirect effect of the interaction of the nanoparticles with cellular components, similar to what was shown previously with polystyrene nanoparticles [37].”
• Mechanisms of nanotoxicity: Generation of reactive oxygen species (2014, background): “[73] Xia T, Kovochich M, Brant J, et al.”
• Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. (2009, background): “8 mmol g −1 as particles size decreases from 300 to 11 nm (ref”
• Progress of zinc oxide‐based nanocomposites in the textile industry (2021, methodology): “Solid phase method ZnSO4·7H2O and anhydrous Na2CO3 Reaction conditions are relatively mild, and output efficiency is high It is difficult to grind sufficiently [28, 29]”
• Nanoparticle-induced unfolding of fibrinogen promotes Mac-1 receptor activation and inflammation. (2011, background): “The pro-inflammatory properties of different nanomaterials have been reported previously, often linked with the ability to induce oxidative stres”
• Understanding biophysicochemical interactions at the nano-bio interface. (2009, background): “In addition to entry into endosomal compartments, engineered nanoparticles exert important effects on other organelle”
• Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms (2008, background): “One exception is nanoparticulate TiO2, which has been shown to generate high levels of peroxide, although these ROS levels have not been shown to be toxic [18] and nanoparticulate TiO2 was not toxic to any of the organisms in the present study.”
• Toxicity of silver nanoparticles in zebrafish models (2008, background): “Cellular deposition of nanoparticles could have exerted stress in the form of oxidative stress [22], DNA damage [23] or proliferation delay [24].”
• Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. (2012, background): “Furthermore, in one study nTiO2 in microbial growth medium induced the generation of O2 • ,(5) but in another study under similar biotic conditions it did not.(2) Previous studies reported that nCeO2 suppressed ROS production and protected cells against oxidant injury.”