Plasma lysozyme levels in sheatfish Silurus glanis (L.) subjected to stress and experimental infection with Edwardsiella tarda

By D. Caruso, O. Schlumberger, C. Dahm, J. Proteau. 2002

93 citations recorded by the source corpus.

Two experiments were conducted to assess plasma lysozyme levels, leucocrit and haematocrit in juvenile sheatfish (90–140 g) under experimentally induced stress and infection. Stress was provoked by random modification of environmental light for 15 min, 16 times a day with a halogen lamp (500 W) and by holding fish in a net for 3 min twice a day. In the first trial, repeated measuring of marked sheatfish showed a progressive significant decrease in plasma lysozyme level, a significant increase in leucocrit and a significant decrease in haematocrit. After a rest period of 1 week, only the leucocrit value was back to the prestress value. In the second trial, light-induced stress did not lead to any variation in lysozyme, leucocrit or haematocrit values. However, after intraperitoneal injection of Edwardsiella tarda (7 × 108 cfu), a strong increase in plasmatic lysozyme in both stressed and unstressed groups of sheatfish was observed. The increase was significant vs. prechallenge levels 3 and 6 days after contamination, but no significant difference was detected between stressed and unstressed fish, despite the strongest stress (light+handling stress) applied to the fish at the beginning of contamination. Decreases in haematocrit values were observed in both groups of fish. A clear increase in leucocrit appeared in unstressed fish, whereas stressed fish showed a significant decrease in leucocrit values. Condition factor was significantly decreased by all kinds of stress. There were no difference in mortality between stressed and unstressed groups.

Subfields: Aquatic Science, Immunology, Nature and Landscape Conservation

View Paper: https://www.semanticscholar.org/paper/4f4d7f51cfc1d0789269b88a247e6dc70d881de0

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Citations in the corpus

Voracious invader or benign feline? A review of the environmental biology of European catfish Silurus glanis in its native and introduced ranges (2009, background): “1988) with Aeromonas species (Farkas and Oláh 1982); flavobacterium species (Farkas 1985), a pasteurella-like bacterium (Farkas and Oláh 1984) and Edwardsiella tarda (Caruso et al. 2002).”
Dissolved Oxygen Level and Stocking Density Effects on Growth, Feed Utilization, Physiology, and Innate Immunity of Nile Tilapia, Oreochromis niloticus (2014, methodology): “Lysozyme activity of fish sera was determined by turbidometric assays as described by Caruso et al. (2002).”
Dietary probiotics and prebiotics improved food acceptability, growth performance, haematology and immunological parameters and disease resistance against Aeromonas hydrophila in snakehead (Channa striata) fingerlings (2014, unknown): “) challenged with Edwardsiella tarda (Caruso et al., 2002).”
Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system (2006, methodology): “Lysozyme activity of fish plasma and skin mucus were determined by turbidometric assays.(21,22) Plasma (100 μL) was added to 900 μL of Micrococcus lysodeikticus (lyophilized cell, Sigma-ALDRICH Chemical company, St.”