A whole-genome assembly of the domestic cow, Bos taurus

By Aleksey V. Zimin, Arthur L. Delcher, Liliana Florea, David R. Kelley, Michael C. Schatz, Daniela Puiu, Finnian Hanrahan, Geo Pertea, C. V. Van Tassell, Tad Stewart Sonstegard and 5 more. Genome Biology, 2009

1,125 citations recorded by the source corpus.

BackgroundThe genome of the domestic cow, Bos taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods.ResultsWe have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate and complete than alternative versions.ConclusionsBy using independent mapping data and conserved synteny between the cow and human genomes, we were able to construct an assembly with excellent large-scale contiguity in which a large majority (approximately 91%) of the genome has been placed onto the 30 B. taurus chromosomes. We constructed a new cow-human synteny map that expands upon previous maps. We also identified for the first time a portion of the B. taurus Y chromosome.

Subfields: Genetics

View Paper: https://www.semanticscholar.org/paper/4e0127b4ba54ada429d55979df1227d6de9e146d

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In Genetics

Citations in the corpus

Building a livestock genetic and genomic information knowledgebase through integrative developments of Animal QTLdb and CorrDB (2018, background): “Whole-genome assemblies for cattle ((1,2), chicken (3), pig (4), sheep (5), horse (6), catfish (7), rainbow trout (8) and other agricultural species have become available within the last 15 years.”
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