Processing of Structurally Heterogeneous Cryo-EM Data in RELION.

By Sjors H. W. Scheres. Methods in Enzymology, 2016

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Integrating model simulation tools and cryo‐electron microscopy (2022, methodology): “Information is typically extracted from this ensemble in one of two ways: first, classification methods can be integrated into the reconstruction process, allowing the 3D reconstruction of multiple conformations from the same set of single-particle images.(10,11) Second, computational methods are de”
3D Variability Analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM (2020, methodology): “The predominant method currently used to resolve heterogeneity in cryo-EM data is discrete 3D classification [13, 40, 42, 43].”
Cryo-EM structures of human hepatitis B and woodchuck hepatitis virus small spherical subviral particles (2022, methodology): “S. H. Scheres, Processing of structurally heterogeneous cryo-EM data in RELION.”
Helical reconstruction in RELION (2016, unknown): “Variations in local resolution were estimated using phase-randomisation in combination with sliding a soft spherical mask across the entire volume (Fernandez-Leiro and Scheres, 2016a).”
Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation (2020, methodology): “S. H. Scheres, Processing of structurally heterogeneous cryo-EM data in RELION.”