Helical reconstruction in RELION
By Shaoda He, Sjors H. W. Scheres. bioRxiv, 2016
413 citations recorded by the source corpus.
We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users. Although our implementation does not solve the problem that grossly incorrect structures can be obtained when the wrong helical symmetry is imposed, we show for four different test cases that it is capable of reconstructing structures to near-atomic resolution. Abbreviations CARD Caspase Activation and Recruitment Domain EM Electron Microscopy FSC Fourier Shell Correlation IHRSR Iterative Helical Real-Space Reconstruction MAVS Mitochondrial Antiviral Signaling Protein TMV Tobacco Mosaic Virus
Subfields: Materials Chemistry, Radiation, Structural Biology
View Paper: https://www.semanticscholar.org/paper/55c56ff20ee7ad73588cb45356fc927401a88eb4
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Materials Chemistry
- Dari Kimanius
- Erik Lindahl
- Björn Forsberg
- Takanori Nakane
- W. Hagen
- Jasenko Zivanov
- Sjors H. W. Scheres
- Alexey G. Murzin
In Radiation
- W. Hagen
- Erik Lindahl
- Takanori Nakane
- Jasenko Zivanov
- Björn Forsberg
- Sjors H. W. Scheres
- Dari Kimanius
In Structural Biology
- Dari Kimanius
- Takanori Nakane
- Erik Lindahl
- Björn Forsberg
- Sjors H. W. Scheres
- W. Hagen
- Jasenko Zivanov
- A. Kotecha
Citations in the corpus
- Structure-based Classification of Tauopathies (2021, positive): “All subsequent image-processing steps were performed using helical reconstruction methods in RELION (58,59).”
- New tools for automated high-resolution cryo-EM structure determination in RELION-3 (2018, positive): “In addition, its functionality was expanded with the incorporation of sub-tomogram averaging ( Bharat et al., 2015 ) and helical reconstruction ( He and Scheres, 2017 ).”