Mass spectrometry in high-throughput proteomics: ready for the big time

By T. Nilsson, Matthias Mann, R. Aebersold, John R. Yates III, Amos Bairoch, J. Bergeron. Nature Methods, 2010

472 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/47a97a1d579c9bdc399f9a1255e9ababcc7a84e2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. R. Aebersold
2. Nicholas T. Ingolia
3. Jonathan S. Weissman
4. Eric W. Deutsch
5. Alexey I. Nesvizhskii
6. B. Domon
7. L. Lareau
8. Cathy H. Wu

In Spectroscopy

1. R. Aebersold
2. Eric W. Deutsch
3. B. Domon
4. Cathy H. Wu
5. Yan V. Sun
6. Yijuan Hu
7. Alexey I. Nesvizhskii
8. Matthias Mann

Citations in the corpus

• Integrative Analysis of Multi-omics Data for Discovery and Functional Studies of Complex Human Diseases (2016, background): “Proper procedures in sample collection, sample preparation, MS experiments and data analysis are all critical to obtain high-quality data for hypothesis-driven or proteome-wide discovery research (Nilsson et al., 2010).”
• High-throughput proteomics: a methodological mini-review (2022, background): “Mass spectrometry (MS) has been developed as one of the most essential and popular tools to identify proteins and their isoforms, and quantify posttranslational modi fi cations, either via the fragments directly or the speci fi c proteolytic activity responsible for their formation 13 – 15 .”
• The Human Proteome Project: Current State and Future Direction (2011, background): “We concluded that recent substantial advances in proteomic technologies including quantitative mass spectrometry, protein capture with antibodies, and bioinformatics for global exchange of large primary data sets and databases make the generation of such a human proteome map feasible (2, 3).”
• Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes (2011, background): “Although there have been remarkable advances in quantitative mass spectrometry (Nilsson et al., 2010), it is difficult to match the large dynamic range and comprehensive nature of deep sequencing.”
• Mapping Intact Protein Isoforms in Discovery Mode Using Top Down Proteomics (2011, unknown): “Together with the careful curation of the Swiss-Prot database 6 , the result is an informatics framework that maps each given protein identification to a single gene (except in rare cases like ubiquitin where multiple genes can produce the identical sequence).”