The Phagosome Proteome
By J. Garin, R. Diez, S. Kieffer, Jean-François Dermine, S. Duclos, É. Gagnon, R. Sadoul, Christiane Rondeau, M. Desjardins. Journal of Cell Biology, 2001
705 citations recorded by the source corpus.
Phagosomes are key organelles for the innate ability of macrophages to participate in tissue remodeling, clear apoptotic cells, and restrict the spread of intracellular pathogens. To understand the functions of phagosomes, we initiated the systematic identification of their proteins. Using a proteomic approach, we identified >140 proteins associated with latex bead–containing phagosomes. Among these were hydrolases, proton pump ATPase subunits, and proteins of the fusion machinery, validating our approach. A series of unexpected proteins not previously described along the endocytic/phagocytic pathways were also identified, including the apoptotic proteins galectin3, Alix, and TRAIL, the anti-apoptotic protein 14-3-3, the lipid raft-enriched flotillin-1, the anti-microbial molecule lactadherin, and the small GTPase rab14. In addition, 24 spots from which the peptide masses could not be matched to entries in any database potentially represent new phagosomal proteins. The elaboration of a two-dimensional gel database of >160 identified spots allowed us to analyze how phagosome composition is modulated during phagolysosome biogenesis. Remarkably, during this process, hydrolases are not delivered in bulk to phagosomes, but are instead acquired sequentially. The systematic characterization of phagosome proteins provided new insights into phagosome functions and the protein or groups of proteins involved in and regulating these functions.
Subfields: Immunology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/4595778533d8e294783b9296a6fa0c9e8ec9d163
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Citations in the corpus
- TLR9 signals after translocating from the ER to CpG DNA in the lysosome (2004, background): “It has been shown in a proteomics approach that, unexpectedly, phagosomes contain several ER-resident protein”
- Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli (2002, background): “A recent analysis identified greater than 160 phagosome-associated proteins in mouse macrophage”
- Treating metastatic cancer with nanotechnology (2011, methodology): “Phagosomes tend to fuse with lysosomes, however, resulting in the degradation of the contents; thus, strategies must be used to control the route of uptak”
- Phagosomes are competent organelles for antigen cross-presentation (2003, background): “4g ) proposes that hydrolases (H) acquired sequentially during phagosome maturation initiate the processing of exogenous peptides in the phagosome lume”
- The Vegetative Vacuole Proteome of Arabidopsis thaliana Reveals Predicted and Unexpected Proteinsw⃞ (2004, background): “…vesicles formed by an inward folding of the cell membrane to hold foreign matter taken into the cell by phagocytosis; occurs before fusion with the lysosome) have demonstrated the presence of plasma membrane, mitochondrial, cytoplasmic, and ER proteins in these preparations (Garin et al., 2001).”
- Galectin-3: an open-ended story. (2006, background): “Another secretory pathway of galectin-3 has been recently implicated; galectin-3 was shown to be a component of exosomes secreted by dendric cells [164,165].”
- Matters Arising Quantitative and Dynamic Assessment of the Contribution of the ER to Phagosome Formation (2005, background): “The original hypothesis of ER-mediated phagocytosis stemmed from biochemical determinations of the protein composition of purified phagosomes (Desjardins et al., 1994a; Garin et al., 2001).”