Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil?
By Qing‐Lin Chen, Xinli An, Hu Li, Yong-guan Zhu, Jianqiang Su, Li Cui. 2017
190 citations recorded by the source corpus.
Abstract Manure application is a common practice that not only adds nutrients and organic matter to arable soils for crop growth, but also introduces antibiotic resistance genes (ARGs), posing a potential risk to human health. To investigate the mechanisms underlying the spread of ARGs in manured soil, especially the impact of manure-borne and indigenous soil microorganisms, a microcosm experiment with four specially designed treatments over a period of two months was conducted, including soil, soil with irradiated pig manure, irradiated soil with pig manure, and soil with pig manure. A total of 240 unique ARGs were detected via a high-throughput quantitative PCR (HT-qPCR) targeting almost all major classes of ARGs. Manure application significantly increased the diversity and abundance of ARGs in soil ( P
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