Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies.

By T. Han, Masato Kato, S. Xie, Leeju C. Wu, Hamid Mirzaei, Jimin Pei, Min Chen, Yang Xie, Jeffrey D. Allen, Guanghua Xiao and 1 more. Cell, 2012

768 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4566dadd7a26baeef5ae0e040ec9674749e44378

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In Molecular Biology

Citations in the corpus

Complexity of the Alternative Splicing Landscape in Plants[C][W][OPEN] (2013, background): “Although a few recent studies point to an important role for AS in plant development and stress responses, functional analysis of individual mRNA isoforms is necessary to understand the importance of AS in plant growth, development, and, especially, stress responses.”
A molecular grammar governing the driving forces for phase separation of prion-like RNA binding proteins (2018, positive): “Our experiments reinforce previous in vitro observations that isolated PLDs of the FET proteins have very different phase behavior than the full-length proteins (Burke et al., 2015; Han et al., 2012; Lin et al., 2015).”
Residue-by-residue view of in vitro FUS granules that bind the C-terminal domain of RNA polymerase II (2015, positive): “Taken together, these results support the view that the RNA-dependence of FUS phase separation can be enhanced by FUS-RNA contacts but that the RNA contacts are not primarily mediated by the LC domain (Han et al., 2012; Kato et al., 2012; Kwon et al., 2013).”
Converging mechanisms in ALS and FTD: Disrupted RNA and protein homeostasis (2013, positive): “…(Polymenidou and Cleveland, 2011 ), which may predispose them to formation of pathological inclusions through their prion-like domains (Kato et al., 2012; Han et al., 2012b; Kim et al., 2013), independent of any proposed progression from an initiating stress granule complex (Dewey et al., 2012).”
FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions (2018, background): “FUS undergoes rapid, physiologically reversible phase separation between dispersed, liquid droplet, and hydrogel states (Han et al., 2012; Kato et al., 2012; Murakami et al., 2015; Patel et al., 2015).”
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation. (2015, positive): “Recent reports show that the prion-like LC domains of FUS can polymerize into fibrous amyloid-like assemblies in a cellfree system (Han et al., 2012; Kato et al., 2012; Kwon et al., 2013, 2014).”
Formation and Maturation of Phase Separated Liquid Droplets by RNA Binding Proteins (2015, background): “These Fus filaments contain a cross-beta structure, similar to classical amyloid filaments formed by the amyloid beta protein (Kato et al., 2012), suggesting that granules may consist of fiber-containing hydrogels (Kato et al., 2012; Han et al., 2012).”
Pi-Pi contacts are an overlooked protein feature relevant to phase separation (2018, background): “Within the testing set, there exist some proteins which have not been shown to be capable of independent phase separation (Han et al., 2012), and which may associate with phase-separated bodies without sharing the same behavior.”
Cell-Free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers Within Hydrogels (2012, methodology): “A central focus of the accompanying manuscript (Han et al., 2012) deals with molecular characterization of the RNA species selectively precipitated by the b-isox microcrystals.”
Liquid-liquid phase separation in biology. (2014, background): “Another is the formation of structures by RNA-binding proteins (Han et al. 2012, Kato et al. 2012, Kwon et al. 2013, Schwartz et al. 2013).”