Complexity of the Alternative Splicing Landscape in Plants[C][W][OPEN]
By Anireddy S. N. Reddy, Yamile Marquez, M. Kalyna, Andrea Barta. The Plant Cell, 2013
752 citations recorded by the source corpus.
Alternative splicing (AS) of precursor mRNAs (pre-mRNAs) from multiexon genes allows organisms to increase their coding potential and regulate gene expression through multiple mechanisms. Recent transcriptome-wide analysis of AS using RNA sequencing has revealed that AS is highly pervasive in plants. Pre-mRNAs from over 60% of intron-containing genes undergo AS to produce a vast repertoire of mRNA isoforms. The functions of most splice variants are unknown. However, emerging evidence indicates that splice variants increase the functional diversity of proteins. Furthermore, AS is coupled to transcript stability and translation through nonsense-mediated decay and microRNA-mediated gene regulation. Widespread changes in AS in response to developmental cues and stresses suggest a role for regulated splicing in plant development and stress responses. Here, we review recent progress in uncovering the extent and complexity of the AS landscape in plants, its regulation, and the roles of AS in gene regulation. The prevalence of AS in plants has raised many new questions that require additional studies. New tools based on recent technological advances are allowing genome-wide analysis of RNA elements in transcripts and of chromatin modifications that regulate AS. Application of these tools in plants will provide significant new insights into AS regulation and crosstalk between AS and other layers of gene regulation.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/7cd79ed6aa0d0b00268e736d4398cffd89665e9c
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses[OPEN] (2013, background): “High-throughput RNA-seq has had a major impact on AS research in plant science, as it has allowed the identification of previously unknown transcript isoforms and assessment of dynamic changes in the full complement of transcript isoforms during development or in response to environmental cues (Webe”