Rapid Detection and Quantification of Members of the Archaeal Community by Quantitative PCR Using Fluorogenic Probes

By Kensaku Takai, K. Horikoshi. Applied and Environmental Microbiology, 2000

852 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing (2014, methodology): “Prokaryote 16S rRNA Pro341F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTACGGGAGGCAGCAGCCTACGGGNBGCASCAG PCR for NGS Modified Takai [23]”
Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. (2005, background): “Three additional primer and probe sets targeting prokaryotes and the domains Archaea and Bacteria, as reported earlier (Amann et al., 1990; Raskin et al., 1994b; Takai and Horikoshi, 2000), were also modified, and denoted PRK, ARC, and BAC, respectively.”
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. (2009, methodology): “To circumvent such challenges, an adaptation of the PCR method developed by Holland et al. (1991) utilizing the so-called ‘50 nuclease assay’ was applied to quantify target 16S rRNA genes amplified from environmental DNA by PCR (Becker et al., 2000; Suzuki et al., 2000; Takai & Horikoshi, 2000).”