Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana.

By C. Chuang, Elliot M. Meyerowitz. Proceedings of the National Academy of Sciences of the United States of America, 2000

722 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/44c0f66cc6332d343d70b71fb3cff0a0bc61cc35

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Citations in the corpus

RNA Interference: Biology, Mechanism, and Applications (2003, background): “Generally, the sense and antisense components of the above-mentioned transgenes are separated only by an intron to increase the efficacy of PTGS (43, 198).”
Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, background): “Unfortunately, the specificity of plant siRNA action has not been studied systematically at the molecular level, although a few papers suggest that moderately closely related homologs are usually not targeted (Chuang and Meyerowitz, 2000; Li et al., 2004; http://www.pi.csiro.au/ rnai/benefits.htm).”
Genetic containment of forest plantations (2007, background): “…containing an inverted repeat or hairpin sequence corresponding to a transcribed region of the target gene has been effective in a variety of plants, and posttranscriptional suppression has been shown to be stably inherited over several generations (Chuang and Meyerowitz 2000; Wesley et al. 2001).”
Fertile Hypomorphic ARGONAUTE (ago1) Mutants Impaired in Post-Transcriptional Gene Silencing and Virus Resistance (2002, background): “These dsRNAs can be supplied exogenously (RNAi) or produced by sense transgenes (cosuppression, quelling) when they are arranged as an inverted repeat (Waterhouse et al., 1998; Chuang and Meyerowitz, 2000; Smith et al., 2000) or when they produce aberrant RNA that serves as a matrix for the RdRP enc”
Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis[W][OA] (2006, result): “While some of the original publications suggested near 100% efficiency of hairpin constructs (Chuang and Meyerowitz, 2000; Wesley et al., 2001), other publications indicate more variable effects (Kerschen et al.”
Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. (2002, background): “We will study the function of these stress-inducible transcription factors using knock-out mutants and transgenics, including overexpression (Jaglo-Ottosen et al., 1998; Kasuga et al., 1999; Liu et al., 1998); antisense suppression (Huang et al., 1999; Nanjo et al., 1999); and double-stranded RNA in”