Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors.
By Mihai G. Netea, N. Gow, C. Munro, S. Bates, C. Collins, G. Ferwerda, R. Hobson, G. Bertram, H. Hughes, Trees Jansen and 11 more. Journal of Clinical Investigation, 2006
755 citations recorded by the source corpus.
The fungal pathogen Candida albicans has a multilayered cell wall composed of an outer layer of proteins glycosylated with N- or O-linked mannosyl residues and an inner skeletal layer of beta-glucans and chitin. We demonstrate that cytokine production by human mononuclear cells or murine macrophages was markedly reduced when stimulated by C. albicans mutants defective in mannosylation. Recognition of mannosyl residues was mediated by mannose receptor binding to N-linked mannosyl residues and by TLR4 binding to O-linked mannosyl residues. Residual cytokine production was mediated by recognition of beta-glucan by the dectin-1/TLR2 receptor complex. C. albicans mutants with a cell wall defective in mannosyl residues were less virulent in experimental disseminated candidiasis and elicited reduced cytokine production in vivo. We concluded that recognition of C. albicans by monocytes/macrophages is mediated by 3 recognition systems of differing importance, each of which senses specific layers of the C. albicans cell wall.
Subfields: Epidemiology, Food Science, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/449eab7597241aff2886044313168875f9cfb862
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Epidemiology
In Infectious Diseases
Citations in the corpus
- Dectin-1 is required for host defense against Pneumocystis carinii but not against Candida albicans (2007, background): “albicans indeed has a multilayered cell wall composed of an outer layer containing N - and O -linked mannosyl residues and an inner skeletal layer of β-glucans and chitin”
- The cell wall: a carbohydrate armour for the fungal cell (2007, background): “Moreover, the localization of the polysaccharides on the cell wall has some direct implication for the fungal cell life (Netea et al., 2006).”
- Dectin-1 is required for β-glucan recognition and control of fungal infection (2006, background): “Enhanced fungal dissemination in dectin-1-knockout mice Because candida is one of the leading causes of nosocomial fungal infections and is well studied in animal”