Consistent Sets of Spectrophotometric Chlorophyll Equations for Acetone, Methanol and Ethanol Solvents

By Raymond J. Ritchie. Photosynthesis Research, 2006

1,257 citations recorded by the source corpus.

Subfields: Industrial and Manufacturing Engineering, Organic Chemistry, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/441fa43070060f1c9ee34c3fc65b53eb6518da26

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Citations in the corpus

Morphology, composition, production, processing and applications of Chlorella vulgaris: A review (2014, methodology): “Analyses and quantification of pigments are conducted by high performance liquid chromatography (HPLC) and spectrophotometry using specific equations [143] or by plotting the calibration curve for each pigment.”
Effect of salinity on growth, biochemical composition, and lipid productivity of Nannochloropsis oculata CS 179 (2012, methodology): “Concentrations of chlorophyll a were determined according to the formulas of Ritchie [26]: [Chlorophyll a] μgmL−1 − 8.0962 A652 + 16.5169 A665 (1) Absorbencies at 652 nm and 665 nm were corrected from turbidity by subtracting absorbency at 750 nm.”
Universal chlorophyll equations for estimating chlorophylls a, b, c, and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents (2008, methodology): “Chl a and d algorithms have recently been published (Ritchie 2006).”
Restoring soil functions by means of cyanobacteria inoculation: Importance of soil conditions and species selection (2018, methodology): “Chlorophyll a concentration was then calculated by applying the ethanol solvent equations reported by Ritchie (2006) [Equation (1)]: Chlorophyll a μ g soil−1 ¼ 11:9035 x A 665−750ð Þ x Vð Þ=g soil−1x L; (1) where A is the absorbance value at the specific wavelength, V is the volume of the extract…”
Remarkably high and consistent tolerance of a Red Sea coral to acute and chronic thermal stress exposures (2021, methodology): “Chl a concentrations were measured in duplicate for each sample based on absorbances at 630 nm, 664 nm, and 750 nm, using a multiscan spectrum spectrophotometer (Biotek HT Synergy plate reader; after Ritchie 2006).”
Differential effects of ocean acidification on growth and photosynthesis among phylotypes of Symbiodinium (Dinophyceae) (2011, methodology): “Chl a concentration was quantified with the use of the equations of Ritchie (2006) and normalized to cell number.”
Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria (2015, positive): “…470 nm, 665 nm and 720 nm (Note 5). b. Calculate concentration of chlorophyll a content according to equations: Chla [µg/ml] = 12.9447 (A665 − A720) (Ritchie, 2006) Chla [µM] = 14.4892 (A665 − A720); for Chla molar mass = 893.4890 g/mol Carotenoids [µg/ml] = [1,000 (A470 − A720) − 2.86 (Chla…”
Ocean acidification enhances the growth rate of larger diatoms (2014, methodology): “Pigment content and elemental composition—Chl a and c were estimated according to Ritchie (2006).”
Development of the polysaccharidic matrix in biocrusts induced by a cyanobacterium inoculated in sand microcosms (2017, methodology): “Chlorophyll a concentration was calculated by applying the formula reported by Ritchie (2006), taking into account the amount of crust (g crust) that was treated: Chla mg L−1 ¼ 11:9035xA 665cð Þ½ xV gcrustð ÞxL where V is the volume of the extract and L is the optical path length.”
Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination (2011, methodology): “…to point out that we limited our formal analysis to a specific set of calculations that were simultaneously developed for multiple solvents (Ritchie, 2006), however, Please cite this article in press as: Castle, S.C., et al., Extraction of chlorophyll a from biological soil crusts: A...,…”
Effect of dissolved oxygen concentration on microalgal culture in photobioreactors (2019, positive): “Three replicates were prepared, and chlorophyll a , b and photoprotective carotenoids (PPC) concentrations were determined using the following relationships [24]: (2) PPC methanol culture 480 750 (3)”