Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

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Denitrification from nitrogen-fixing biologically crusted soils in a cool desert environment, southeast Utah, USA (2013, methodology): “Chla was extracted with ethanol (Castle et al. 2011) and assessed spectrophotometrically (Beckman DU-64, Beckman Instruments Inc., Fullerton, CA). Soil texture was determined using a modified rapid method procedure described by Kettler et al. (2001), which involved removing carbonates and organic ma”
Production of greenhouse‐grown biocrust mosses and associated cyanobacteria to rehabilitate dryland soil function (2016, methodology): “Likewise, the chlorophyll a values found in the most productive treatments were similar to those found in naturally occurring well-developed crusts containing a mix of light and dark cyanobacteria, as well as mosses and lichens (Castle et al. 2011).”
Development of the polysaccharidic matrix in biocrusts induced by a cyanobacterium inoculated in sand microcosms (2017, methodology): “Chlorophyll a in biocrusts was determined as reported by Castle et al. (2011): 1 g of homogenized crust was treated with 5 mL ethanol at 80 °C for 5 min and then incubated at 4 °C for 8 h. Subsequently, samples were centrifuged at 3500×g for 15 min and the pigment-containing supernatant was…”