Immunoaffinity profiling of tyrosine phosphorylation in cancer cells

By John Rush, A. Moritz, Kimberly A. Lee, A. Guo, V. Goss, E. Spek, Hui Zhang, Xiangming Zha, R. Polakiewicz, M. Comb. Nature Biotechnology, 2005

1,146 citations recorded by the source corpus.

Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/41fc3ac9dde7ea04184e74b7ea2d3761ebbffd38

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Matthias Mann
2. Jüergen Cox
3. Stefka Tyanova
4. John R. Yates III
5. Jesper V. Olsen
6. Steven P. Gygi
7. Florian Gnad
8. C. Ruse

In Radiology, Nuclear Medicine and Imaging

1. Matthias Mann
2. Ji Luo
3. Stephen J. Elledge

In Spectroscopy

1. Matthias Mann
2. Jüergen Cox
3. John R. Yates III
4. Stefka Tyanova
5. Jesper V. Olsen
6. Florian Gnad
7. C. Ruse
8. A. Nakorchevsky

Citations in the corpus

• Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. (2007, methodology): “In that study, SILAC (reviewed in Mann [2006]) and peptide immunoprecipitation (Rush et al., 2005) using phosphospecific antibodies followed by mass spectrometry before and after DNA damage was used to identify those proteins that were inducibly phosphorylated on SQ or TQ motifs.”
• Proteomics by mass spectrometry: approaches, advances, and applications. (2009, background): “Compared with the serine and threonine phosphorylation, investigation of the phosphotyrosine proteome relies almost exclusively on immunoaffinity purifications (208).”
• The Fundamental Flaws of Immunoassays and Potential Solutions Using Tandem Mass Spectrometry (2009, methodology): “Enrichment for specific post-translational modifications has also been carried out using solid phase strategies after protein digestion (Rush et al., 2005).”
• Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. (2014, methodology): “For tyrosine inhibition of phosphatases, cells were treated with 1 mM pervanadate and 50 ng/ml calyculin A for 15 min at 37 C as described earlier (Rush et al., 2005).”
• Erasers of histone acetylation: the histone deacetylase enzymes. (2014, background): “Because HDAC1-Y221 (HDAC2-Y222) is conserved in humans, mice, Xenopus laevis, and Caenorhabditis elegans, tyrosine phosphorylation may also be important in the regulation of HDAC1 and HDAC2 activities.”
• Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. (2007, positive): “…and Analysis by LC-MS/MS Mass Spectrometry Phosphopeptide immunoprecipitation from different cell lines was performed as described previously(Rush et al., 2005) using the PhosphoScan Kit (P-Tyr-100) from CST. Analysis of Phosphorylation Site Data Sets To assign peptide sequences, we used…”
• An iterative statistical approach to the identification of protein phosphorylation motifs from large-scale data sets (2005, methodology): “However, refinements of several affinity-based strategies such as immunoaffinit”
• PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse (2011, background): “The numbers of discovered phospho-Tyr, ubiquitinyl-Lys and acetyl-Lys have been greatly amplified by the availability of appropriate affinity reagents (9,13–15).”