OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis

By Maike C Rentel, D. Lecourieux, F. Ouaked, Sarah L. Usher, L. Petersen, H. Okamoto, Heather Knight, Scott C. Peck, C. Grierson, Heribert Hirt and 1 more. Nature, 2004

602 citations recorded by the source corpus.

Active oxygen species (AOS) generated in response to stimuli and during development can function as signalling molecules in eukaryotes, leading to specific downstream responses. In plants these include such diverse processes as coping with stress (for example pathogen attack, wounding and oxygen deprivation), abscisic-acid-induced guard-cell closure, and cellular development (for example root hair growth). Despite the importance of signalling via AOS in eukaryotes, little is known about the protein components operating downstream of AOS that mediate any of these processes. Here we show that expression of an Arabidopsis thaliana gene (OXI1) encoding a serine/threonine kinase is induced in response to a wide range of H2O2-generating stimuli. OXI1 kinase activity is itself also induced by H2O2 in vivo. OXI1 is required for full activation of the mitogen-activated protein kinases (MAPKs) MPK3 and MPK6 after treatment with AOS or elicitor and is necessary for at least two very different AOS-mediated processes: basal resistance to Peronospora parasitica infection, and root hair growth. Thus, OXI1 is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/3ff75369fcd0bb9e97b87579688b1b22fc05a93d

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Citations in the corpus

Plant immunity: the EDS1 regulatory node. (2005, background): “Other Arabidopsis genetic components that link ROI molecules to downstream stress responses have recently been uncovered [31,32].”
Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications (2009, background): “The enzyme is a member of the AGC protein kinase family and was originally identified because its expression was induced by H2O2 in vivo (Rentel et al., 2004).”
Signal transduction during cold, salt, and drought stresses in plants (2011, neutral): “response to ROS stress [141, 145].”
Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. (2006, background): “MPK3 and MPK6 activities in ROS signaling are affected by an Arabidopsis serine/threonine kinase, OXI1, whose kinase activity is induced by H2O2 [43].”
Redox Regulation in Photosynthetic Organisms: Signaling, Acclimation, and Practical Implications (2009, positive): “While a relatively large number of genes have been identiﬁed as singlet oxygen-inducible (11, 236, 307), the mechanism of signal transduction is unknown.”
The molecular mechanism of zinc and cadmium stress response in plants (2012, background): “Heavy metal stress-induced ROS production and accumulation in plant cells is detected by the OXI1 (Oxidative Signal-Inducible 1) protein for H2O2, and the EX1 (Executer 1) or EX2 proteins for superoxide [188, 189].”
Nitric oxide, stomatal closure, and abiotic stress. (2007, background): “These include the protein kinases OST1 (Mustilli et al., 2002) and OXI1 (Rentel et al., 2004) and many other as yet unknown proteins and signalling molecules.”
Hydrogen peroxide involvement in formation and development of adventitious roots in cucumber (2007, background): “Although H2O2 is involved in the growth of lateral roots (Su et al. 2006) and the formation of root hairs (Foreman et al. 2003; Rentel et al. 2004), it is a novel finding that H2O2 can promote the formation and development of adventitious roots in cucumber.”
Signaling mechanisms in pattern-triggered immunity (PTI). (2015, background): “and DAMPs such as treatment with flg22, cellulase, and H2O2 in leaves and roots (Rentel et al., 2004; Jacobs et al., 2011).”