Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.

By Dahlene N. Fusco, N. Accornero, B. Lavoie, S. Shenoy, J. Blanchard, R. Singer, E. Bertrand. Current Biology, 2003

597 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3f869b3b8805871b57478ec93f167fc7a1fa3367

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In Molecular Biology

1. M. Saxton
2. A. Senecal
3. Jeetayu Biswas
4. Maria Vera
5. H. Park
6. Y. Shav-Tal
7. R. Singer
8. Ravi Sachidanandam

Citations in the corpus

• Inaugural Article: Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function (2010, unknown): “The absolute numbers given above, although objective and useful in distinguishing gross differences in behavior (51, 53), are not precisely robust or generalizable because they depend heavily on chosen thresholds and tests.”
• A protein tagging system for signal amplification in gene expression and fluorescence imaging (2014, unknown): “A commonly used method to study RNA localization, even at the single-molecule level, is to insert multiple copies (as many as 24) of the MS2-binding RNA hairpin into a target RNA molecule, which then recruits many MS2-GFP fusion proteins, fluorescently labeling the RNA molecule with many GFP molecul”
• From silencing to gene expression: real-time analysis in single cells. (2004, methodology): “MS2-YFP was expressed from plasmids with either CMV quence elements in the eukaryotic genome such that or RNA polymerase II large subunit promoters (Fusco et al., 2003) (Supplemental Data available on Cell website). pSV2-EYFP-Suv39heritable gene expression patterns are ensured. h1-C1 (mouse) and…”
• Xist nucleates local protein gradients to propagate silencing across the X chromosome (2021, unknown): “pMS2-GFP (Fusco et al., 2003) Addgene plasmid cat #: 27121”
• SINGLE-PARTICLE TRACKING (2009, background): “The behavior of mRNP in the cytoplasm was different [146]: 33–40% was completely immobile; 15–25% was freely diffusing; 40–41% showed corralled diffusion; and 2–5% showed rapid directional movement along microtubules.”
• Transcriptional pulsing of a developmental gene. (2006, background): “The MS2 RNAMS2 protein interaction has a nanomolar affinity and is highly stable, as cytoplasmic RNA particles retain bound MS2-GFP even if the unbound fraction is restricted to the nucleus [21].”
• Single-Cell and Single-Molecule Analysis of Gene Expression Regulation (2016, background): “mRNA molecules exhibit complex dynamics switching between diffusive motion and directional transport (52), which can be analyzed by using a software for inferring transient transport states (75, 101).”
• Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “This problem can be partially solved by low expression levels of FP or its targeting to the nucleus (114).”