Improving the photostability of bright monomeric orange and red fluorescent proteins

By Nathan Christopher Shaner, Michael Z. Lin, Michael R Mckeown, Paul A. Steinbach, Kristin L. Hazelwood, Michael W. Davidson, Roger Tsien. Nature Methods, 2008

1,040 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3f82413928245f966152bf5aadc5611dcc206878

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biophysics

In Molecular Biology

Citations in the corpus

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum (2013, background): “Fusions of mNeonGreen with signal peptides and targeting proteins confirmed expected localization patterns in the cytoskeleton (β-actin, Lifeact, fascin, cortactin, plastin (fimbrin), MAP Tau, light chain myosin, myosin IIA, EB3, TPX2 and myotilin), intermediate filaments (keratin and vimentin), the”
A Guide to Fluorescent Protein FRET Pairs (2016, neutral): “Engineering bright FPs with high photostability has been challenging due to the lack of rational methods for predicting the effects of speciﬁc mutations on photostability, although limiting oxygen access to the FP’s chromophore has been proposed as a mechanism for improving photostability [45,63].”
Sensitive red protein calcium indicators for imaging neural activity (2016, methodology): “R-GECO1 and RCaMP1 are based on circularly permuted mApple (Shaner et al., 2008) and mRuby (Kredel et al.”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “Enhanced versions were reported recently for both proteins, although tradeoffs remain: faster maturation of mKO2 seems to be accompanied by lower pH stability (370), and photostable mOrange2 matures slower than the original protein (385).”
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, background): “These properties seem to be inherited from the mApple fluorescent protein, which shows similar photoswitching behavior (Shaner et al., 2008).”
Direct stochastic optical reconstruction microscopy with standard fluorescent probes (2011, background): “On the contrary, PA-FPs as used in PALM show a lower photostability and brightness than standard organic fluorophore”
Two-photon absorption properties of fluorescent proteins (2011, neutral): “0 [78] [28] [42] [43] tdTomato(41) 582 581 581 557 554 553 3.”
Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins (2014, methodology): “Therefore, by calculating pre: post stimulation ratio of TagRFP-T fluorescence intensity within the ROI, we determined the relative fold-change of binding in the light and dark.”
A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (2011, methodology): “The amplified cDNAs were cloned into mammalian expression vectors in frame with a RFP (in the case of Hip1R, tDimer [65]; in the case of myosin1E, mApple [77]; and for all other proteins mCherry [78]; see Table S2) to generate either N- or C-terminal fusion proteins upon expression.”