Roles for Arabidopsis CAMTA Transcription Factors in Cold-Regulated Gene Expression and Freezing Tolerance[W][OA]

By Colleen J. Doherty, Heather A Van Buskirk, Susan J. Myers, M. Thomashow. The Plant Cell Online, 2009

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The Arabidopsis thaliana CBF cold response pathway plays a central role in cold acclimation. It is characterized by rapid cold induction of genes encoding the CBF1-3 transcription factors, followed by expression of the CBF gene regulon, which imparts freezing tolerance. Our goal was to further the understanding of the cis-acting elements and trans-acting factors involved in expression of CBF2. We identified seven conserved DNA motifs (CM), CM1 to 7, that are present in the promoters of CBF2 and another rapidly cold-induced gene encoding a transcription factor, ZAT12. The results presented indicate that in the CBF2 promoter, CM4 and CM6 have negative regulatory activity and that CM2 has both negative and positive activity. A Myc binding site in the CBF2 promoter was also found to have positive regulatory effects. Moreover, our results indicate that members of the calmodulin binding transcription activator (CAMTA) family of transcription factors bind to the CM2 motif, that CAMTA3 is a positive regulator of CBF2 expression, and that double camta1 camta3 mutant plants are impaired in freezing tolerance. These results establish a role for CAMTA proteins in cold acclimation and provide a possible point of integrating low-temperature calcium and calmodulin signaling with cold-regulated gene expression.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/1b8c5f49ca2ecb6e285bee2d334d948db1cfac2e

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Citations in the corpus

Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis[W][OA] (2012, background): “Cold stress activates CBF expression (Gilmour et al., 1998; Liu et al., 1998) and induces the expression of a large subset of COR genes (Maruyama et al., 2004; Vogel et al., 2005), and the overexpression of CBFs leads to constitutively enhanced freezing tolerance (Jaglo-Ottosen et al., 1998; Liu et ”
Early abscisic acid signal transduction mechanisms: newly discovered components and newly emerging questions. (2010, background): “Analysis of synthetic promoters suggests that, although a single ABRE may be insufficient, two copies of the ABRE or a single ABRE combined with the related ABRE-coupling element (ABRE-CE; ACGCGT/G/C) renders a promoter ABA-sensitive (Hobo et al. 1999; Zhang et al. 2005).”
Regulation of water, salinity, and cold stress responses by salicylic acid (2014, background): “…which encodes a calmodulin-binding transcription activator, recognizes the CBF2/DREB1C promoter to positively regulate the expression of CBF2/DREB1C to enhance cold tolerance (Doherty et al., 2009), contributes to the up-regulation of 15% of the cold-inducible genes (Kim et al., 2013).”
Genomics of Cold Hardiness in Woody Plants (2014, background): “AtCBF1 is activated by the MYC-like bHLH INDUCER OF CBF EXPRESSION 2 (AtICE2) via an unknown promoter element (Furosova et al., 2009) and apparently also by the calmodulin binding transcription activator CAMTA3 (Doherty et al., 2009).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “The expression of CBFs is repressed by MYB15, a MYB family transcriptional repressor (Agarwal et al., 2006).”
High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation[C][W][OA] (2011, background): “This motif has been shown to bind CAMTA TFs (Yang and Poovaiah, 2002) involved in signaling responses to wounding, cold, and other stresses (Walley et al., 2007; Doherty et al., 2009).”
Calcium Signals: The Lead Currency of Plant Information Processing (2010, background): “Important evidence for a direct link between Ca2+ signaling via CAMTA1 and CAMTA3 and the regulation of cold tolerance in plants was provided by the discovery that these CAMTA proteins bind to regulatory elements in the promoter region of the DREB1c/CBF2 gene (Doherty et al., 2009).”
Advances in physiological and molecular aspects of plant cold tolerance (2017, positive): “Calmodulin is a calciumbinding protein which binds to a regulatory element in the CBF2 gene promoter which plays a role in controlling the CBF regulon and freezing tolerance (Doherty et al. 2009).”
Advances in Plant Tolerance to Abiotic Stresses (2016, background): “For instance, CBL1 has been shown to regulate cold response by interacting with CIPK7 [99], whereas CAMTA3 has been identified as a positive regulator of CBF2/DREB1C through binding to a regulatory element (CG-1, vCGCGb) in its promoter [100].”
Molecular Basis of Plant Cold Acclimation: Insights Gained from Studying the CBF Cold Response Pathway1 (2010, background): “As mentioned above, the regulation of CBF1 and CBF2 involves action of the calmodulin-binding CAMTA transcription factors (Doherty et al., 2009).”
Overview of Cold Stress Regulation in Plants (2021, background): “The CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR proteins act directly involment in the transduction process of low temperature-induced cytosolic calcium signals into downstream regulation (Doherty et al. 2009).”