Protein production by auto-induction in high density shaking cultures.
By F. Studier. 2005
5,919 citations recorded by the source corpus.
Subfields: Biotechnology, Genetics, Materials Chemistry
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In Biotechnology
In Genetics
In Materials Chemistry
- Barry L. Stoddard
- Paul Murphy
- David Baker
- Jasmine L. Gallaher
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Citations in the corpus
- Zn2+ Inhibits Coronavirus and Arterivirus RNA Polymerase Activity In Vitro and Zinc Ionophores Block the Replication of These Viruses in Cell Culture (2010, methodology): “In short, E. coli BL21(DE3) with plasmid pDEST14-nsp9-CH was grown in auto-induction medium ZYM-5052 [47] for 6 hours at 37uC and a further 16 hours at 20uC.”
- Structural basis for cooperative function of Mettl3 and Mettl14 methyltransferases (2016, neutral): “Target proteins were expressed in cultures grown in autoinduction media at 20 C overnight (Studier, 2005).”