Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity

By A. Nostro, M. P. Germanò, V. D'Angelo, A. Marino, M. A. Cannatelli. Letters in Applied Microbiology, 2000

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Medicinal plants and antimicrobial activity. (2005, methodology): “For their part,Nostro et al. (2000)demonstrated that species suchHelichrysum italicumor Phytolacca dodecandra showed moderate activity against Escherichia coliwhen the diethyl extract obtained after extraction of the aqueous suspension of the drug powder was used, but that there were no active extra”
Techniques for evaluation of medicinal plant products as antimicrobial agents: Current methods and future trends (2010, methodology): “Some researchers impregnate the paper disk with plant extract before putting on the inoculated plates (Lourens et al., 2004; Salie et al., 1996) while others prefer after (Nostro et al., 2000; Baris et al., 2006).”
Screening methods to determine antibacterial activity of natural products (2007, methodology): “Some researchers (5,13) employed 2 mg/mL salt solution, others preferred to use a 5 mg/mL solution (9).”