Transcriptional Landscape of Human Tissue Lymphocytes Unveils Uniqueness of Tumor-Infiltrating T Regulatory Cells

By M. de Simone, Alberto Arrigoni, G. Rossetti, P. Gruarin, V. Ranzani, Claudia Politano, R. Bonnal, Elena Provasi, M. L. Sarnicola, I. Panzeri and 21 more. Immunity, 2016

604 citations recorded by the source corpus.

Summary Tumor-infiltrating regulatory T lymphocytes (Treg) can suppress effector T cells specific for tumor antigens. Deeper molecular definitions of tumor-infiltrating-lymphocytes could thus offer therapeutic opportunities. Transcriptomes of T helper 1 (Th1), Th17, and Treg cells infiltrating colorectal or non-small-cell lung cancers were compared to transcriptomes of the same subsets from normal tissues and validated at the single-cell level. We found that tumor-infiltrating Treg cells were highly suppressive, upregulated several immune-checkpoints, and expressed on the cell surfaces specific signature molecules such as interleukin-1 receptor 2 (IL1R2), programmed death (PD)-1 Ligand1, PD-1 Ligand2, and CCR8 chemokine, which were not previously described on Treg cells. Remarkably, high expression in whole-tumor samples of Treg cell signature genes, such as LAYN, MAGEH1, or CCR8, correlated with poor prognosis. Our findings provide insights into the molecular identity and functions of human tumor-infiltrating Treg cells and define potential targets for tumor immunotherapy.

Subfields: Immunology, Oncology

View Paper: https://www.semanticscholar.org/paper/3c04bc522d941ecaafbc597e1e476aaecf780563

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In Immunology

1. W. Nicholas Haining
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4. Yuanyuan Zhang
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Citations in the corpus

• Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing. (2017, background): “(A) The Venn graph showing the overlap of tumor Treg genes identified in this study with those from previous studies by De Simone et al. (2016) (p = 2e-85), Plitas et al. (2016) (p = 6e-129), and Tirosh et al. (2016) (p = 2.1e-141), determined by hypergeometric test.”
• CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation (2017, background): “The digested NFATc3 and His-GB1 were separated and further purified using size exclusion chromatography (GE Healthcare Life Sciences “Superdex 75 10/300 GL”).”