A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling

By I. Kratchmarova, Shao-En Ong, M. Nielsen, L. Foster, Matthias Mann. Nature Biotechnology, 2003

725 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/3b2ffd62b33af71326f5c57db9dc434a2975b5f6

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Citations in the corpus

Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway*S (2005, methodology): “We and another group have described stable isotope labelling by amino acids in cell culture (termed SILAC and SIL, respectively) as a generic quantitative proteomics technology [26-28], and applied SILAC to determine signal induced multiprotein complexes, protein-protein interactions and the time or”
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (2006, background): “…ª2006 Elsevier Inc. 635 downstream protein kinases and their substrates (Hunter, 2000; Pawson and Nash, 2003), and several MS studies have focused on tyrosine-phosphorylation ‘‘early events’’ after growth-factor treatment (Blagoev et al., 2003, 2004; Kratchmarova et al., 2005; Zhang et al., 2005).”
The beginning of a beautiful friendship: cross-linking/mass spectrometry and modelling of proteins and multi-protein complexes (2010, background): “This approach is well established in proteomics and relies on incorporating stable isotopes, for example by amino acids in cell culture (SILAC) (Ong et al., 2002, 2003; Blagoev et al., 2003).”
Phosphotyrosine interactome of the ErbB-receptor kinase family (2005, background): “The approach taken in this study is thus complementary to other proteomic approaches in which whole signaling complexes are identified (see, e.g., Blagoev et al, 2003).”
Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics (2004, background): “but also for differential labeling of protein populations in functional experiment”
Computational prediction of proteotypic peptides for quantitative proteomics (2007, background): “, revealed new components and changes in the composition of protein complexes and organelle”
Protein interaction networks from yeast to human. (2004, background): “Also, many smaller regions of protein interaction networks were explored in detail, such as the EGF (epidermal growth factor) signaling pathway [23] and interactions among bZIP proteins [24].”
The abc's (and xyz's) of peptide sequencing (2004, background): “Recent developments now make it possible to determine more transient and signal-dependent interaction”
Mass spectrometry–based proteomics turns quantitative (2005, background): “Quantitative proteomics can break this yin and yang of protein biochemistr”
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) (2006, methodology): “In mammalian culture systems, it has been applied to detect specific biological changes in functional proteomics assay”
Decoding signalling networks by mass spectrometry-based proteomics (2010, background): “SILAC-based quantification of EGF-stimulated versus non-stimulated cells then distinguished specific protein interactors — those binding owing to EGF-mediated EGFR activation — from hundreds of background proteins that bound to the bait or bead”
Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein*S (2005, background): “Recently not only comprehensive quantitation studies between two states (5, 6) but also protein-protein ( 7 , 8), protein-peptide (9), and protein-drug (10) interaction analyses have been reported.”