Spatio-temporal images of growth-factor-induced activation of Ras and Rap1

By N. Mochizuki, Shigeko Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, M. Matsuda. Nature, 2001

602 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3a79db1c5f78c2e1329e76fb9d65161ded243e81

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Molecular Biology

Citations in the corpus

Development of an optimized backbone of FRET biosensors for kinases and GTPases (2011, methodology): “The substrate peptide of human CrkII (a.a. 217–225) was used as the sensor domain. pRaichu-Ras and Raichu-Rac1 containing an EV linker were constructed by replacing the linker region of the original biosensors with an EV linker (Mochizuki et al., 2001; Itoh et al., 2002).”
Interferon-α induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6 (2004, methodology): “Plasmids encoding fusion proteins IRF7-CFP, IRF3-CFP and MyD88-YFP were constructed essentially as describe”
Cytochrome c binds to inositol (1,4,5) trisphosphate receptors, amplifying calcium-dependent apoptosis (2003, background): “3, is comparable with values obtained for numerous other interacting protein”
Activation of Rac and Cdc42 Video Imaged by Fluorescent Resonance Energy Transfer-Based Single-Molecule Probes in the Membrane of Living Cells (2002, methodology): “The occurrence of FRET in the living cells was confirmed by a photobleaching experiment as described previously ( 36 ).”
FRET or no FRET: a quantitative comparison. (2003, background): “The model is applicable, for example, in the case where a donor and an acceptor dye molecule are coupled to two different domains of a molecule and variations in FRET efficiencies represent conformational changes (Suzuki et al., 1998; Mochizuki et al., 2001).”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “The universality and relative simplicity of such a design have led to the development of numerous FRETbased sensors of this type, including GES for monitoring changes in the concentration of Ca(2) (130, 153, 262, 263, 291, 292, 295, 304, 307, 327, 434), cyclic nucleotides (174, 317, 318, 375, 445, 5”
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. (2008, background): “Many genetically encoded indicators that utilize Green Fluorescent Protein (GFP)-based Fluorescence Resonance Energy Transfer (FRET), including cameleon (Miyawaki et al., 1997) and Raichu-Ras (Mochizuki et al., 2001), are distributed in the cytoplasm.”