Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

By Jan Lötvall, Andrew F. Hill, F. Hochberg, Edit I. Buzás, D. Di Vizio, C. Gardiner, Y. Gho, I. Kurochkin, Suresh Mathivanan, P. Quesenberry and 5 more. Journal of Extracellular Vesicles, 2014

2,497 citations recorded by the source corpus.

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3a182846a65a8c8eb898fb7de4f8e7fb835d2c41

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Hina Kalra
2. C. Théry
3. E. Batrakova
4. M. Kim
5. Suresh Mathivanan
6. P. Vader
7. G. Drummen
8. Jan Lötvall

In Molecular Biology

1. C. Théry
2. Hina Kalra
3. Suresh Mathivanan
4. E. Batrakova
5. G. Drummen
6. M. Kim
7. Jan Lötvall
8. P. Vader

Citations in the corpus

• exoRBase: a database of circRNA, lncRNA and mRNA in human blood exosomes (2017, background): “Notably, before submitting, users should check whether their study meets the standards proposed by ISEV (International Society for Extracellular Vesicles) (25).”
• Exosome-Based Cell-Cell Communication in the Tumor Microenvironment (2018, methodology): “…in the field, the International Society for Extracellular Vesicles recently released guidelines for the analysis of EVs and the reporting of the results (Lotvall et al., 2014), a standardization initiative followed by others, such as the EV-TRACK Consortium (EV-TRACK Consortium et al., 2017).”
• Optimized exosome isolation protocol for cell culture supernatant and human plasma (2015, background): “Furthermore, the purity of exosome isolations is important and can be classified through a number of methods, including the presence of contaminating extracellular proteins (24).”
• Focus on Extracellular Vesicles: Introducing the Next Small Big Thing (2016, methodology): “However, such a standardization is also only possible when genuine and unique markers for different types of EVs can be identified [24].”
• Exosomes in cancer: small particle, big player (2015, background): “The International Society for Extracellular Vesicles (ISEV) has recently released minimal experimental requirements for definition of extracellular vesicles and their functions [21].”
• Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake (2016, neutral): “It must be emphasized that there is some controversy on nomenclature and sizes of the different types of vesicles (Gould and Raposo 2013; Witwer et al. 2013); however, basic requirements of criteria for EVs have been established (Lötvall et al. 2014).”
• ExoCarta: A web-based compendium of exosomal cargo (2015, neutral): “Exosomes are small (30-150 nm) membranous vesicles secreted by a variety of cells into the extracellular microenvironment [1, 2].”
• Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey (2016, neutral): “At this time, there is no consensus on a ‘‘gold standard’’ method for EVisolation and purification (5).”
• Manufacturing Exosomes: A Promising Therapeutic Platform. (2018, neutral): “The International Society of Extracellular Vesicles (ISEV) has established a set of criteria for the proteomic identification of exosomes with a minimal list of requirements [76], namely, exosomes should (i) have transmembrane proteins to provide evidence of amembrane (e.”