Functional engraftment of human ES cell–derived dopaminergic neurons enriched by coculture with telomerase-immortalized midbrain astrocytes

By N. Roy, C. Cleren, S. K. Singh, Lichuan Yang, M. Beal, Steven A. Goldman. Nature Medicine, 2006

832 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Developmental Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/39ebd21f9c487ad524bb05702d37d128f72178bc

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. M. Lundblad
2. V. Tabar
3. M. Beal
4. G. Keller
5. O. Lindvall
6. L. Studer
7. D. Surmeier

In Developmental Neuroscience

1. V. Tabar
2. G. Keller
3. O. Lindvall
4. L. Studer
5. D. Surmeier

In Molecular Biology

1. Charles E. Murry
2. G. Keller
3. R. Jaenisch
4. C. Beard
5. George W. Bell
6. F. Soldner
7. M. Lundblad
8. V. Tabar

Citations in the corpus

• Generation of regionally specified neural progenitors and functional neurons from human embryonic stem cells under defined conditions. (2012, background): “Despite the short culture time prior to transplantation, the grafts showed no signs of tumor formation or necrosis, which are features otherwise commonly associated with transplantation of progenitors derived from pluripotent stem cells (Miura et al., 2009; Roy et al., 2006).”
• Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. (2008, background): “For example, transplanting a population of human ESC neural derivatives enriched for dopamine neurons into Parkinsonian rats resulted in a primitive population of nestin neuroepithelial cells that continued to proliferate in the striatum 70 days after transplantation (Roy et al., 2006).”
• Floor plate-derived dopamine neurons from hESCs efficiently engraft in animal models of PD (2011, background): “There are also considerable safety concerns for PSCs related to their potential for teratoma formation or neural overgrowt”
• Parkinson’s Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors (2009, methodology): “Generation of Dopaminergic Neurons from PD Patient-Derived hiPSCs Several protocols for the directed differentiation of hESCs into dopaminergic neurons have been established (Elkabetz et al., 2008; Kim et al., 2006; Perrier et al., 2004; Roy et al., 2006; Sonntag et al., 2007).”
• Ethical and Safety Issues of Stem Cell-Based Therapy (2018, background): “Primitive population of nestin+ neuroepithelial cells, that continued to proliferate in the striatum, was noticed in rats with Parkinson disease, 70 days after transplantation of hESC-derived dopamine neurons [19].”
• Donor-Derived Brain Tumor Following Neural Stem Cell Transplantation in an Ataxia Telangiectasia Patient (2009, background): “Recently the use of human ESC-derived dopaminergic neurons enriched by coculture with telomerase-immortalized midbrain astrocytes resulted in undifferentiated expansion suggesting there was a potential for tumor development when injected to rodents [14].”