Simultaneous whole-animal 3D-imaging of neuronal activity using light-field microscopy

By R. Prevedel, Young-Gyu Yoon, M. Hoffmann, N. Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Ed Boyden and 1 more. Nature Methods, 2014

769 citations recorded by the source corpus.

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

Subfields: Biomedical Engineering, Biophysics, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/39c24ee8d515c0296e450d84417055c4dde0dcac

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Citations in the corpus

Could a Neuroscientist Understand a Microprocessor? (2016, background): “Clearly there is a lot of structure in this spatiotemporal dataset.”
Closed-loop and activity-guided optogenetic control. (2015, positive): “Electrical/Optical Devices Enabling Closed-Loop Control in Rodents and Primates In the papers described above, digitized electrophysiological measurements provided a readily utilizable, submillisecond output source for closed-loop optogenetic control because real-time systems already exist for elect”
A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging (2016, methodology): “We specified a collection NA of 1.0 (specifications listed Figure 1C).”
Light Field Image Processing: An Overview (2017, positive): “spatial multiplexing has also been applied to capture specimens at micro-scales [7], [57], [58], [59].”
Simultaneous multi-plane imaging of neural circuits (2016, neutral): “Despite their high performance, wide-field imaging schemes such as light-sheet microscopy (Ahrens et al., 2013), light field microscopy (Prevedel et al., 2014), or swept confocally-aligned planar excitation (SCAPE) (Bouchard et al., 2015), suffer from light scattering in deep tissues and are thus…”
OpenWorm: an open-science approach to modeling Caenorhabditis elegans (2014, methodology): “…the worm, such as the demonstration of dynamic “mind control” of C. elegans via direct laser stimulation of individual neurons expressing optogenetic components (Leifer et al., 2011) and simultaneous whole-animal 3D imaging of neuronal activity using light-ﬁeld microscopy (Prevedel et al., 2014).”
Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging Data (2016, positive): “Recent technical advances have enabled complete imaging of small cortical volumes (e.g., Cotton et al., 2013), and whole-brain imaging of small animals (Ahrens et al., 2013; Prevedel et al., 2014) at reasonable imaging rates.”
Compressive light-field microscopy for 3D neural activity recording (2016, background): “Light-field microscopy has already provided promising results for functional brain imaging [20] with 3D volume image reconstructions to quantify the fluorescence levels of individual neurons.”