Simultaneous multi-plane imaging of neural circuits

By Weijian Yang, Weijian Yang, J. K. Miller, Luis Carrillo-Reid, Eftychios A. Pnevmatikakis, Liam Paninski, Rafael Yuste, Darcy S. Peterka. 2016

220 citations recorded by the source corpus.

Summary Recording the activity of large populations of neurons is an important step towards understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain.

Subfields: Biophysics, Cognitive Neuroscience

Citations in the corpus

• Re-evaluating circuit mechanisms underlying pattern separation (2019, neutral): “Several promising new imaging technologies that can record intracellular calcium from large numbers of identified cells have also been recently developed (Grewe et al., 2011; Katona et al., 2012; Froudarakis et al., 2014; Nadella et al., 2016; Sofroniew et al., 2016; Yang et al., 2016).”
• Simultaneous Denoising, Deconvolution, and Demixing of Calcium Imaging Data (2016, unknown): “…in scattering tissue, or artificially, due to the simultaneous observation of the projection of multiple z planes through spatial light modulation (Yang et al., 2015, companion paper), or more general projection patterns as suggested and studied in Pnevmatikakis and Paninski (2013); such…”