Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

By J. Livet, T. Weissman, Hyuno Kang, R. W. Draft, Ju Lu, Robyn Bennis, J. Sanes, Jeff William Lichtman. 2007

1,858 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Neurology

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

In Molecular Biology

In Neurology

Citations in the corpus

Structural and molecular interrogation of intact biological systems (2013, neutral): “(6) Immediately place the tissue (for example, brain) in 20 ml cold hydrogel solution in a 50-ml conical tube.”
Complex brain networks: graph theoretical analysis of structural and functional systems (2009, background): “…including a tendency for synaptic connections to be reciprocal and clustered. a promising approach for mapping connectivity involves the stochastic expression of several fluorescent proteins 40 , and this may ultimately deliver a complete map of the cellular interconnections of an entire brain 41 .”
Tracking adult stem cells (2011, methodology): “The original Brainbow mice were designed to allow colour-coding of neurons with up to 90 distinguishable colour codes”
Three-dimensional intact-tissue sequencing of single-cell transcriptional states (2018, neutral): “S15); STARmap may also be capable of integrating cell-type information with single-neuron morphology and projection anatomy (for example, by means of Brainbow and MAPseq) (43, 44) as well as with in vivo neural activity imaging and electrophysiology.”
Genetic dissection of neural circuits. (2008, positive): “This limitation has recently been overcome in the Brainbow mice by expression of distinct mixtures of XFPs in individual neurons (Livet et al., 2007).”
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011, methodology): “Methods for labeling specific cell types regardless of tissue depth and geometry have rapidly progressed with the advent of genetically encoded fluorescent proteins and transgenic marking methods such as Brainbo”
The human connectome: Origins and challenges (2013, background): “In 2007, Jeff Lichtman and colleagues wrote about “connectomic maps” which they defined as “connectivity maps in which multiple, or even all, neuronal connections are rendered” (Livet et al., 2007, pg. 56).”
Soft materials in neuroengineering for hard problems in neuroscience. (2015, background): “…for cellular-level brain imaging in freely moving animals (Flusberg et al., 2008; Ghosh et al., 2011), and transgenic multicolor labeling strategies for neurons (Livet et al., 2007) represent some of the latest examples in which new technical approaches are enabling fundamental discoveries.”
Review of spectral imaging technology in biomedical engineering: achievements and challenges (2013, background): “show that >90 different neuronal clones could be distinguished in the inventors of Brainbow mice according to color and intensity gathered by combining tissue/temporally inducible Cre recombinase, multiplex recombination sites encompassing multiple fluorescent protein gene cassettes, and spectral co”
Highly multiplexed subcellular RNA sequencing in situ (2014, background): “Using RNA barcodes from expression vectors, one can label up to 4 N ( N =barcode length) cells uniquely, much more than is possible using a combination of fluorescent proteins (19).”
Physiology of Astroglia. (2018, neutral): “The pool of available astroglial promoters is continuously increasing, and several fluorescent protein probes with distinct spectral characteristics are now in use (703, 999).”