Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment

By T. Kaeberlein, Kim Lewis, S. Epstein. Science, 2002

1,231 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/350b4b72e52176bc712168f86579216222720738

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Stuart E. Jones
2. J. Lennon
3. Kim Lewis
4. Amy Spoering
5. E. Stewart
6. N. Kaldalu
7. Iris Keren
8. Devang T. Shah

In Molecular Biology

1. Kim Lewis
2. J. Lennon
3. Stuart E. Jones
4. Amy Spoering
5. N. Kaldalu
6. Iris Keren
7. Devang T. Shah
8. J. Dupré

Citations in the corpus

• The Rebirth of Culture in Microbiology through the Example of Culturomics To Study Human Gut Microbiota (2015, methodology): “In the environment, it is believed that only 1% of bacterial species are cultivable by using current techniques (8).”
• Growing Unculturable Bacteria (2012, background): “The groups of Epstein and Lewis designed a diffusion chamber to accomplish this by enclosing the bacteria within a semipermeable chamber such that the cells are unable to pass through the membrane barrier but nutrients and growth factors from the environment are able to enter (37).”
• Cleaning up with genomics: applying molecular biology to bioremediation (2003, neutral): “Culturing microorganisms directly in the environmen”
• Phylogenetic and Physiological Diversity of Cultured Deep-Biosphere Bacteria from Equatorial Pacific Ocean and Peru Margin Sediments (2007, background): “The majority of prokaryotes in natural environments do not grow on standard laboratory media since the complex conditions of the natural habitat are difficult to reproduce(Amann et al. 1995; Kaeberlein et al. 2002).”
• Size doesn’t matter: towards a more inclusive philosophy of biology (2007, background): “31One reason so few prokaryotes have been cultured may be because laboratory environments provide only nutrients and not signals from community members (Kaeberlein et al. 2002).”
• Microbial seed banks: the ecological and evolutionary implications of dormancy (2011, background): “Novel cultivation strategies have shown that the recovery of microbial taxa can be enhanced by experimenting with enrichment conditions that more realistically simulate environmental condition”
• Multidrug tolerance of biofilms and persister cells. (2008, background): “Unculturable microorganisms are grown in their natural environment in a diffusion chamber (Kaeberlein et al. 2002) and are then used as a source of secondary metabolites for drug discovery.”