In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei

By C. Schaffitzel, I. Berger, J. Postberg, J. Hanes, H. Lipps, A. Plückthun. Proceedings of the National Academy of Sciences of the United States of America, 2001

606 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3484cd7c7cf139f12682d58c3b21fc61b04e3ebf

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Citations in the corpus

G-quadruplexes and their regulatory roles in biology (2015, background): “This permitted the first direct visualization by in situ immuno staining in the micronuclei of the ciliate Stylonychia lemnae (32).”
Selecting and screening recombinant antibody libraries (2005, background): “The most successful applications of ribosome display are in the field of affinity maturation of antibodie”
The Structure and Function of DNA G-Quadruplexes (2020, background): “telomeric G4 structure formation was observed to be dynamically controlled through protein interactions in a cell cycle-dependent manner [39,40].”
QGRS Mapper: a web-based server for predicting G-quadruplexes in nucleotide sequences (2006, background): “The present work focuses only on the unimolecular quadruplexes, since it is more likely to be encountered in physiological conditions (7,8).”
Stability and kinetics of G-quadruplex structures (2008, background): “(251) described the generation of quadruplex-specific single chain antibodies.”
Prevalence of quadruplexes in the human genome (2005, positive): “Quadruplexes have also been shown to exist in vivo in Stylonychia lemnae macronuclei (7).”
Human telomere, oncogenic promoter and 5′-UTR G-quadruplexes: diverse higher order DNA and RNA targets for cancer therapeutics (2007, background): “Strong evidence supporting G-quadruplex formation in vivo comes from the demonstration that in vitro generated single-chain antibody fragments specific for intermolecular telomeric G-quadruplex DNA react with ciliated protozoan Stylonychia lemnaemacronuclei but not corresponding micronuclei (62).”
G-quadruplexes in promoters throughout the human genome (2006, background): “Folding topology is important in the recognition of quadruplexes, as has been demonstrated for both protein (33) and small molecule (34) quadruplex ligands, because different surfaces are presented for recognition.”
Crystal structure of parallel quadruplexes from human telomeric DNA (2002, background): “, which may be involved in the structure of telomere end”
Targeting G-quadruplexes in gene promoters: a novel anticancer strategy? (2011, background): “Most studies on G-quadruplexes have been carried out in ex vivo systems, although the occurrence of these structures in telomeres has been unequivocally demonstrated in ciliate”