Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics.

By Juri Rappsilber, Yasushi Ishihama, Matthias Mann. Analytical Chemistry, 2003

2,656 citations recorded by the source corpus.

Subfields: Computational Mechanics, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/33a280e4a5c427d5dcf5bb69a094eeed36b54e7d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Computational Mechanics

In Spectroscopy

Citations in the corpus

SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. (2008, methodology): “The arrows in (F) and (I) label the reduced peak intensity of b1 and a2 integrin, respectively.”
Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. (2014, methodology): “The (phospho)peptides were desalted on C18 StageTips (Rappsilber et al., 2003).”
In-gel digestion for mass spectrometric characterization of proteins and proteomes (2006, methodology): “Peptide mixtures can also be purified before LC MS/MS on STAGE (Stop and go extraction) tips before loadin”
Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein*S (2005, methodology): “Peptide fractionation by strong cation exchange chromatography (SCX) was performed using SCX-StageTip with 0–500 mM five-step ammonium acetate salt elution (23), and the resultant fractions were desalted using C18 StageTips prior to LC-MS/MS analysis.”
Serum Albumin Domain Structures in Human Blood Serum by Mass Spectrometry and Computational Biology (2015, methodology): “The resulting digests were desalted using self-made C18 StageTips (23) prior to mass spectrometric analysis.”
Parts per Million Mass Accuracy on an Orbitrap Mass Spectrometer via Lock Mass Injection into a C-trap*S (2005, methodology): “The reduced and alkylated protein mixtures were digested and the resulting peptide mixtures were desalted on RP-C18 StageTips as described previously (16) and diluted in 0.”
Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway*S (2005, methodology): “Prior to analysis by mass spectrometry, the samples were desalted with C18 STAGE tips (35).”
Exosomes as Critical Agents of Cardiac Regeneration Triggered by Cell Therapy (2014, methodology): “At 15 and 30 days after injection, global heart function was greater in animals injected with CDC exosomes compared with NHDF exosomes or media controls (Figure 2A).”
Small angle X-ray scattering and cross-linking for data assisted protein structure prediction in CASP 12 with prospects for improved accuracy (2018, methodology): “24 Resulting peptides were desalted using StageTips.”