Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis.

By M. Boter, O. Ruíz-Rivero, Ashraf Abdeen, S. Prat. Genes & Development, 2004

607 citations recorded by the source corpus.

Jasmonates (JA) are important regulators of plant defense responses that activate expression of many wound-induced genes including the tomato proteinase inhibitor II (pin2) and leucine aminopeptidase (LAP) genes. Elements required for JA induction of the LAP gene are all present in the -317 to -78 proximal promoter region. Using yeast one-hybrid screening, we have identified the bHLH-leu zipper JAMYC2 and JAMYC10 proteins, specifically recognizing a T/G-box AACGTG motif in this promoter fragment. Mutation of the G-box element decreases JA-responsive LAP promoter expression. Expression of JAMYC2 and JAMYC10 is induced by JA, with a kinetics that precedes that of the LAP or pin2 transcripts. JAMYC overexpression enhanced JA-induced expression of these defense genes in potato, but did not result in constitutive transcript accumulation. Using footprinting assays, an additional protected element was identified, located directly adjacent to the T/G-box motif. Mutation of this element abolishes JA response, showing that recognition of this duplicated element is also required for gene expression. Knockout mutants in the AtMYC2 homolog gene of Arabidopsis are insensitive to JA and exhibit a decreased activation of the JA-responsive genes AtVSP and JR1. Activation of the PDF1.2 and b-CHI, ethylene/JA-responsive genes, is, however, increased in these mutants. These results show that the JAMYC/AtMYC2 transcription factors function as members of a MYC-based regulatory system conserved in dicotyledonous plants with a key role in JA-induced defense gene activation.

Subfields: Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/333e1396b72a1d5d530274d5a6898d3f8d9a729d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Insect Science

In Plant Science

Citations in the corpus

The Role of Stress-Responsive Transcription Factors in Modulating Abiotic Stress Tolerance in Plants (2020, background): “MYC2 was ident fied in a mutant screen for insensitivity to exogenous JA, and further characterizati revealed that MYC2 is a key component of the JA signali g pathway [71].”
Transcription factors involved in terpenoid indole alkaloid biosynthesis in Catharanthus roseus (2007, background): “In Arabidopsis, the bHLH transcription factor AtMYC2 (also called JIN1 or RAP-1), which binds to the G-box (de Pater et al. 1997), regulates the expression of certain JA-responsive genes (Lorenzo et al. 2004; Boter et al. 2004 ).”
Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. (2006, background): “AtMYC2, which was first identified as a transcriptional activator that is involved in the ABAmediated drought stress signaling pathway [18], upregulates the expression of genes that are involved in the JAmediated wounding response and negatively regulates the expression of JA/ET-mediated pathogen de”
Jasmonate Regulates the INDUCER OF CBF EXPRESSION–C-REPEAT BINDING FACTOR/DRE BINDING FACTOR1 Cascade and Freezing Tolerance in Arabidopsis[W] (2013, background): “The bHLH transcription factors JASMONATE INSENSITIVE1 (JIN1/MYC2), MYC3, and MYC4 function as direct targets of JAZ proteins to modulate a subset of jasmonate-regulated responses, such as the inhibition of root elongation and defense responses (Boter et al., 2004; Lorenzo et al., 2004; Chini et al.,”
Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis (2004, background): “The interaction among JA, ethylene, and ABA signaling is just emerging from this and other recent studies (Boter et al., 2004; Lorenzo et al., 2004).”
Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. (2006, background): “Recently, AtMYC2 was also reported as a transcription factor that functions in JA and JA-ethylene-regulated defense responses in Arabidopsis (3, 7, 70).”
The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses[C][W] (2011, background): “MYC2 is a key bHLH TF regulating the expression of different subsets of JA-responsive genes (Boter et al., 2004; Lorenzo et al., 2004; Dombrecht et al., 2007).”
Plant Defense against Insect Herbivores (2013, background): “(JAZ) are normally bound to transcription factors, such as MYC2, inhibiting their activity [144,145].”
Indirect defence via tritrophic interactions. (2008, background): “These proteins are repressors of MYC2 and similar transcription factors, which are important players in the regulation of jasmonate-sensitive genes (Boter et al., 2004; Lorenzo et al., 2004).”
The role of gibberellin signalling in plant responses to abiotic stress (2014, background): “…that are degraded by the 26S proteasome in the presence of active forms of JA, provides competition for binding of JAZ proteins to the MYC2 transcription factor, one of a number of transcription factors regulating JA-dependent transcriptional responses (Boter et al., 2004; Hou et al., 2010).”
Multiple cis-regulatory elements regulate distinct and complex patterns of developmental and wound-induced expression of Arabidopsis thaliana 4CL gene family members (2006, background): “cis-element AACGTG, the binding site for JAMYC2 and JAMYC10 bHLH transcription factors that direct JA-induced expression in tomato and Arabidopsis (Boter et al. 2004) are found in the promoters of these genes.”
COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine (2008, background): “Arabidopsis JAZ3 (also known as JAI3) in- teracts with and presumably represses the activity of the MYC2 transcription factor that promotes the expression of JAresponsive genes (6).”
MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis[W] (2007, background): “2 (At5g44420); HEL/PR4 (At3g04720); CHI/PR3 (At3g12500); ADC2/ SPE2 (At4g34710); VSP1 (At5g24780); MYB75/PAP1 (At1g56650); TT19/GST12 (At5g17220); PAL1 (At2g37040); EGL3 (At1g63650); ESP (At1g54040); ILR1 (At3g02875); MYB34/ATR1 (At5g60890); DHAR (At1g19570); AOC4 (At1g13280); TDR1 (At3g23230); VSP2”