Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack.

By M. de Vos, Vivian R van Oosten, Remco M. P. van Poecke, Johan A Van Pelt, M. Pozo, Martin J. Mueller, A. Buchala, J. Metraux, L. C. van Loon, M. Dicke and 1 more. Molecular Plant-Microbe Interactions, 2005

1,016 citations recorded by the source corpus.

Plant defenses against pathogens and insects are regulated differentially by cross-communicating signaling pathways in which salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) play key roles. To understand how plants integrate pathogen- and insect-induced signals into specific defense responses, we monitored the dynamics of SA, JA, and ET signaling in Arabidopsis after attack by a set of microbial pathogens and herbivorous insects with different modes of attack. Arabidopsis plants were exposed to a pathogenic leaf bacterium (Pseudomonas syringae pv. tomato), a pathogenic leaf fungus (Alternaria brassicicola), tissue-chewing caterpillars (Pieris rapae), cell-content-feeding thrips (Frankliniella occidentalis), or phloem-feeding aphids (Myzus persicae). Monitoring the signal signature in each plant-attacker combination showed that the kinetics of SA, JA, and ET production varies greatly in both quantity and timing. Analysis of global gene expression profiles demonstrated that the signal signature characteristic of each Arabidopsis-attacker combination is orchestrated into a surprisingly complex set of transcriptional alterations in which, in all cases, stress-related genes are overrepresented. Comparison of the transcript profiles revealed that consistent changes induced by pathogens and insects with very different modes of attack can show considerable overlap. Of all consistent changes induced by A. brassicicola, Pieris rapae, and E occidentalis, more than 50% also were induced consistently by P. syringae. Notably, although these four attackers all stimulated JA biosynthesis, the majority of the changes in JA-responsive gene expression were attacker specific. All together, our study shows that SA, JA, and ET play a primary role in the orchestration of the plant's defense response, but other regulatory mechanisms, such as pathway cross-talk or additional attacker-induced signals, eventually shape the highly complex attacker-specific defense response.

Subfields: Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/32df50afbb089e0991c5b2b29d33b851803faa3e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Insect Science

1. Martin Heil
2. A. Agrawal
3. M. Dicke
4. D. Cipollini
5. C. Pieterse
6. N. V. van Dam
7. G. Howe
8. D. Walters

In Plant Science

1. Martin Heil
2. C. Pieterse
3. M. Dicke
4. D. Walters
5. N. V. van Dam
6. A. Agrawal
7. D. Cipollini
8. M. Pozo

Citations in the corpus

• Plant immunity to insect herbivores. (2008, background): “DNA microarray studies show that the jasmonate pathway has a dominant role in regulating global changes in gene expression in response to both mechanical wounding and herbivory (23, 24, 39, 84, 99, 100, 102, 103).”
• Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration[W][OA] (2006, background): “filtration medium as described ( Cutler et al., 2000 ).”
• Abiotic and Biotic Stress Response Crosstalk in Plants (2011, background): “Similarly, JA signaling is induced in tomato and Arabidopsis by Tetranychus urticae and Pieris rapae, respectably (Li et al., 2002; Reymond et al., 2004; De Vos et al., 2005).”
• Role of plant hormones in plant defence responses (2009, background): “…JA signaling is activated in response to attack by Manduca sexta caterpillars in tobacco (Kahl et al. 2000), spider mite Tetranychus urticae in tomato (Li et al. 2002a) and Pieris rapae caterpillars or Frankliniella occidentalis thrips in Arabidopsis (Reymond et al. 2004; De Vos et al. 2005).”
• MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis[W] (2007, background): “For instance, although both pathogen and insect attacks stimulate JA biosynthesis, most changes in JA-responsive gene expression occur in an attacker-dependent manner (De Vos et al., 2005), suggesting that plants can divert limited resources in the best possible way.”
• Silverleaf Whitefly Induces Salicylic Acid Defenses and Suppresses Effectual Jasmonic Acid Defenses1[W][OA] (2006, background): “Transcriptome analysis after aphid feeding on Arabidopsis further confirmed the trends observed by Moran and Thompson (Moran et al., 2002; De Vos et al., 2005).”
• Plant NBS-LRR proteins: adaptable guards (2006, background): “ing pathways, involving both synergism and mutual antagonism between pathways [88-91].”