Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration[W][OA]

By Monica Stein, Jan Dittgen, Clara Sánchez-Rodríguez, B. Hou, A. Molina, Paul Schulze-Lefert, Volker Lipka, S. Somerville. The Plant Cell Online, 2006

678 citations recorded by the source corpus.

Arabidopsis thaliana is a host to the powdery mildew Erysiphe cichoracearum and nonhost to Blumeria graminis f. sp hordei, the powdery mildew pathogenic on barley (Hordeum vulgare). Screening for Arabidopsis mutants deficient in resistance to barley powdery mildew identified PENETRATION3 (PEN3). pen3 plants permitted both increased invasion into epidermal cells and initiation of hyphae by B. g. hordei, suggesting that PEN3 contributes to defenses at the cell wall and intracellularly. pen3 mutants were compromised in resistance to the necrotroph Plectosphaerella cucumerina and to two additional inappropriate biotrophs, pea powdery mildew (Erysiphe pisi) and potato late blight (Phytophthora infestans). Unexpectedly, pen3 mutants were resistant to E. cichoracearum. This resistance was salicylic acid–dependent and correlated with chlorotic patches. Consistent with this observation, salicylic acid pathway genes were hyperinduced in pen3 relative to the wild type. The phenotypes conferred by pen3 result from the loss of function of PLEIOTROPIC DRUG RESISTANCE8 (PDR8), a highly expressed putative ATP binding cassette transporter. PEN3/PDR8 tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated at infection sites. PEN3/PDR8 may be involved in exporting toxic materials to attempted invasion sites, and intracellular accumulation of these toxins in pen3 may secondarily activate the salicylic acid pathway.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/eb51a96e08853f69308455f189a6b3d81489c83f

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Citations in the corpus

A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety (2007, background): “Furthermore, the powdery mildew non-host resistance-related genes PEN1 , PEN2 and PEN3 [17,18] were found in 5, 5 and 10 copies, respectively.”
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis*S (2007, background): “infection (58), and the phosphorylation sites we identified here”
Plant immunity: towards an integrated view of plant–pathogen interactions (2010, background): “thaliana are involved in preventing cellular penetration by powdery mildew fung”
Regulation and function of root exudates. (2008, background): “…involved in diverse cellular processes, such as the excretion of potential toxic compounds, lipid translocation, heavy metal tolerance, nutrient transport, salt stress and in disease resistance (Balzi & Goffeau 1994; Szczypka et al. 1994; Maathuis et al. 2003; Kobae et al. 2006; Stein et al. 2006).”
A MicroRNA Superfamily Regulates Nucleotide Binding Site–Leucine-Rich Repeats and Other mRNAs[W][OA] (2012, background): “This unidentified protein has similarity to PEN3 that is involved for basal immunity in Arabidopsis (Stein et al., 2006).”
PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid (2010, background): “PDR-type ABC transporters have also been reported to be involved in responses to pathogens (19) and a broad range of stresses, including salinity, cold, and heavy metals”
The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. (2007, background): “pathogen resistance (Kobae et al., 2006; Stein et al., 2006).”
Fungal Pathogens: The Battle for Plant Infection (2006, background): “Unexpectedly, PEN3 mutants are resistant to E. cichoracearum (Stein et al., 2006).”