RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

By J. Yu, S. DeRuiter, D. Turner. Proceedings of the National Academy of Sciences of the United States of America, 2002

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siRNA vs. shRNA: similarities and differences. (2009, background): “be active and it was soon demonstrated that active siRNA consists of a hairpin structure can be transcribed in cells from an RNA polymerase III promoter on a plasmid construct [49,50].”
Ki67 is a promising molecular target in the diagnosis of cancer (review). (2015, background): “In a number of studies, Ki67 appeared to be closely correlated with pancreatic tumor severity as well as with expression of Smac and thus may be useful as a diagnostic and prognostic marker or, in conjunction with Smac, as an indicator of treatment efficacy (73‑75).”
Antisense technologies. Improvement through novel chemical modifications. (2003, methodology): “Alternatively, two short RNA molecules were transcribed separately using two U6 promotors [118,124,125].”
RNA Interference: Biology, Mechanism, and Applications (2003, background): “Several groups have devised strategies to synthesize short RNAs in vitro (64) or by introducing plasmids with the ability to make de novo siRNAs inside the cell (235, 239).”
RNA silencing: Methods and protocols (2006, background): “Bertrand Seraphin (18,19) and is a yeast expression vector that contains a TAP tag consisting of two IgG-binding domains of Staphylococcus aureus protein A and a calmodulinbinding peptide (CBP) separated by a TEV protease cleavage site.”